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PMID: 6104568 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Radioimmunoassay of secretin. A critical review and current status.

Digestive diseases and sciences ·Vol. 25 ·No. 7 ·1980-07-00 ·Pages 529-52

Chang TM, Chey WY

Abstract

The radioimmunoassay methods of secretin are reviewed with respect to production of antibody, preparation of radioactive tracers, and effect of plasma interference. The major difference in the secretin assay methods resides in handling plasma interference. Thus the assay sensitivity decreased markedly when the assay was conducted by diluting plasma samples. When the assay was conducted by compensating for plasma interference with homologous hormone-free plasma, the effect of plasma interference was greatly reduced, leading to a more sensitive assay. However, this method probably can not obtain consistent results with plasma samples collected under various experimental conditions. The method is still subject to considerable desensitization and assay variation. On the other hand, the elimination of plasma interference before assay results in the most sensitive secretin assays capable of detecting consistently a significant postprandial rise in plasma secretin level. It is concluded that a sensitive, validated secretin radioimmunoassay should be one that is capable of detecting increments of plasma secretin in response to doses of intraduodenal acid at 0.055 mEq/min or lower and intravenous administration of exogenous secretin at 0.03 CU/kg/hr with concomitant stimulation of pancreatic bicarbonate and water secretion. With a sensitive and accurate radioimmunoassay for secretin, it is now possible to further investigate the physiology and pathophysiology of secretin.

MeSH Terms
Acids/pharmacology Animals Antibody Affinity Antibody Formation Antibody Specificity Bile Acids and Salts/pharmacology Ethanol/pharmacology Food Humans Intestine, Small/metabolism Iodine Radioisotopes Radioimmunoassay Secretin/blood,immunology,metabolism Somatostatin/pharmacology Vagus Nerve/physiology
Chemicals
Acids Bile Acids and Salts Iodine Radioisotopes Secretin Ethanol Somatostatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chang T M
Chey W Y
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Article Info
Journal
Digestive diseases and sciences
Abbr.
Dig Dis Sci
ISSN
0163-2116
DOI
10.1007/BF01315215
Published
1980-07-00
Pages
529-52
Language
English
Region
United States
NLM ID
7902782
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01315215 View journal details
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