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PMID: 6142077 Published · ppublish English Journal Article

T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4.

The Journal of experimental medicine ·Vol. 159 ·No. 3 ·1984-03-01 ·Pages 716-30

Gunter KC, Malek TR, Shevach EM

Abstract

We have identified a single rat monoclonal antibody, G7, that is a potent inducer of interleukin (IL-2) production from all functioning T cell hybridomas as well as from normal T cells. G7 is also mitogenic for normal T cells and is a very effective inducer of IL-2 receptor expression. On fluorescence-activated cell sorter analysis, G7 recognized a pan-T cell antigen. Immunoprecipitation studies demonstrated that G7 recognized a cell surface molecule of 28-32 kD that appeared to be identical to Thy-1 in coprecipitation studies. In addition, G7 precipitated a protein of 50 kD. The possible relationship of the putative molecular complex identified by G7 on murine cells to the molecular complex identified on human T cells with anti-T3 reagents is discussed. In addition, G7 should prove to be a very useful reagent for studying the early events of lymphocyte activation as well as an inducer of lymphokine-rich supernatants.

MeSH Terms
Animals Antibodies, Monoclonal/immunology,physiology Antigens, Surface/genetics,immunology Antilymphocyte Serum/pharmacology Interleukin-2/biosynthesis Interphase Lymphocyte Activation Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Nude Rats Rats, Inbred Lew Receptors, Immunologic/immunology Receptors, Interleukin-2 T-Lymphocytes/cytology,immunology Thy-1 Antigens
Chemicals
Antibodies, Monoclonal Antigens, Surface Antilymphocyte Serum Interleukin-2 Receptors, Immunologic Receptors, Interleukin-2 Thy-1 Antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gunter K C
Malek T R
Shevach E M
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37 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.159.3.716
Published
1984-03-01
Pages
716-30
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187253
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.159.3.716 PMC Full Text View journal details
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