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PMID: 6142104 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Divalent cations differentially support transmitter release at the squid giant synapse.

The Journal of physiology ·Vol. 346 ·1984-01-00 ·Pages 257-71

Augustine GJ, Eckert R

Abstract

The ability of Ca, Sr and Ba ions to support transmitter release was studied at the squid giant synapse by examining their respective actions on presynaptic current and post-synaptic responses. Transmitter-induced post-synaptic currents were smaller in Sr- than in Ca- containing solutions, and much smaller in Ba-containing solutions. The time course and amplitude of spontaneous miniature post-synaptic potentials were similar in the presence of all three divalent ions. Sr or Ba substitution has little effect on the resting potential of presynaptic terminals. In Sr-containing solutions, action potentials were similar in amplitude and time course to those recorded in Ca. Ba slightly prolonged action potential duration but had no effect on amplitude. Voltage-clamped presynaptic terminals exhibited inward Ca, Sr or Ba currents which were apparently carried through Ca channels. These currents were similar in amplitude and time course in all three ions, being somewhat larger in Ba. Although presynaptic currents were similar in these ions, transmitter release induced by these currents depended upon the divalent species entering the presynaptic terminal. Release was greatest in response to presynaptic current carried by Ca and smallest in response to current carried by Ba. Transfer curves relating presynaptic current to post-synaptic potential were sigmoidal in all three ions, and exhibited limiting slopes of approximately 2. Divalent cations differentially support transmitter release at the squid giant synapse in the sequence Ca greater than Sr much greater than Ba. The differential efficacy of the divalent cations is not due to post-synaptic alterations, presynaptic potential changes or differences in presynaptic divalent cation conductances. This sequence may reflect the cation selectivity of the exocytotic process responsible for transmitter release.

MeSH Terms
Action Potentials/drug effects Animals Barium/pharmacology Calcium/pharmacology Decapodiformes In Vitro Techniques Ion Channels/physiology Membrane Potentials/drug effects Neurotransmitter Agents/metabolism Strontium/pharmacology Synapses/drug effects,metabolism,physiology Synaptic Transmission/drug effects
Chemicals
Ion Channels Neurotransmitter Agents Barium Calcium Strontium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Augustine G J
Eckert R
References (64)
64 references, click to expand
  1. Interaction of barium ions with potassium channels in squid giant axons.
    Biophys J. 1980 Jun;30(3):473-88 PMID: 6266531
  2. Extracellular potassium and trasmitter release at the giant synapse of squid.
    J Physiol. 1977 Apr;266(2):209-18 PMID: 16128
  3. The role of the alkaline earth ions in anaphylactic histamine secretion.
    J Physiol. 1972 Aug;224(3):753-69 PMID: 4116105
  4. Presynaptic calcium currents in squid giant synapse.
    Biophys J. 1981 Mar;33(3):289-321 PMID: 7225510
  5. Effects of strontium ions on end-plate channel properties.
    J Physiol. 1980 Sep;306:567-77 PMID: 6257899
  6. Barium and strontium can substitute for calcium in noradrenaline output induced by excess potassium in the guinea-pig.
    J Physiol. 1980 Aug;305:59-71 PMID: 6255149
  7. Miniature synaptic potentials at the squid giant synapse.
    J Neurobiol. 1978 Jul;9(4):329-35 PMID: 210256
  8. On the role of barium in supporting the asynchronous release of acetylcholine quanta by motor nerve impulses.
    J Physiol. 1978 Jan;274:157-71 PMID: 304889
  9. Presynaptic calcium diffusion and the time courses of transmitter release and synaptic facilitation at the squid giant synapse.
    J Neurosci. 1983 Jun;3(6):1263-9 PMID: 6133920
  10. L-glutamate blockade of transmission at the giant synapse of the squid stellate ganglion.
    J Neurobiol. 1969;1(2):209-19 PMID: 4334647
  11. Influx of calcium, strontium, and barium in presynaptic nerve endings.
    J Gen Physiol. 1982 Jun;79(6):1065-87 PMID: 6286843
  12. Nickel and calcium ions modify the characteristics of the acetylcholine receptor-channel complex at the frog neuromuscular junction.
    J Physiol. 1980 Feb;299:203-18 PMID: 6247480
  13. Rate-limiting step of inhibitory post-synaptic current decay in Aplysia buccal ganglia.
    J Physiol. 1980 Jul;304:145-64 PMID: 6255140
  14. Effects of some divalent cations on synaptic transmission in frog spinal neurones.
    J Physiol. 1979 Sep;294:387-406 PMID: 229215
  15. On the factors which determine the amplitude of the miniature end-plate potential.
    J Physiol. 1957 Jul 11;137(2):267-78 PMID: 13449877
  16. Tetraethylammonium contains an impurity which alkalizes cytoplasm and reduce calcium buffering in neurons.
    Brain Res. 1981 Mar 16;208(2):473-8 PMID: 6260296
  17. The action of calcium on neuronal synapses in the squid.
    J Physiol. 1966 May;184(2):473-98 PMID: 5921841
  18. Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.
    J Cell Biol. 1979 May;81(2):275-300 PMID: 38256
  19. Equivalence of Ca2+ and Sr2+ in transmitter release from K+-depolarised nerve terminals.
    Nature. 1979 Nov 1;282(5734):84-5 PMID: 41184
  20. The function of the proximal synapses of the squid stellate ganglion.
    J Gen Physiol. 1959 Jan 20;42(3):609-16 PMID: 13620890
  21. Role of presynaptic calcium ions and channels in synaptic facilitation and depression at the squid giant synapse.
    J Physiol. 1982 Feb;323:173-93 PMID: 6284915
  22. Axon voltage-clamp simulations. III. Postsynaptic region.
    Biophys J. 1975 Jan;15(1):37-54 PMID: 169925
  23. Membrane currents carried by Ca, Sr, and Ba in barnacle muscle fiber during voltage clamp.
    J Gen Physiol. 1974 May;63(5):564-78 PMID: 4824996
  24. Voltage clamp experiments in striated muscle fibres.
    J Physiol. 1970 Jul;208(3):607-44 PMID: 5499787
  25. Strontium and quantal release of transmitter at the neuromuscular junction.
    J Physiol. 1969 Jan;200(1):267-83 PMID: 4387376
  26. The statistical nature of the acetycholine potential and its molecular components.
    J Physiol. 1972 Aug;224(3):665-99 PMID: 5071933
  27. Measurement of calcium influx under voltage clamp in molluscan neurones using the metallochromic dye arsenazo III.
    J Physiol. 1979 Jan;286:61-82 PMID: 439040
  28. Calcium channel.
    Annu Rev Neurosci. 1981;4:69-125 PMID: 6261668
  29. The effect of voltage on the time course of end-plate currents.
    J Physiol. 1972 May;223(1):151-71 PMID: 4537943
  30. Equilibrium potential for the postsynaptic response in the squid giant synapse.
    J Gen Physiol. 1974 Nov;64(5):519-35 PMID: 4374500
  31. Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse.
    Biophys J. 1981 Mar;33(3):323-51 PMID: 6261850
  32. Transmitter release induced by injection of calcium ions into nerve terminals.
    Proc R Soc Lond B Biol Sci. 1973 Jul 3;183(1073):421-5 PMID: 4147099
  33. The effects of strontium and barium ions at synapses in sympathetic ganglia.
    J Physiol. 1977 May;267(2):497-518 PMID: 195042
  34. Transmission by presynaptic spike-like depolarization in the squid giant synapse.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2415-9 PMID: 6954549
  35. Calcium currents in internally perfused nerve cell bodies of Limnea stagnalis.
    J Physiol. 1982 Jan;322:503-28 PMID: 7069629
  36. Spontaneous synaptic potentials and quantal release of transmitter in the stellate ganglion of the squid.
    J Physiol. 1967 Sep;192(2):379-406 PMID: 4383088
  37. A further study of the statistical composition on the end-plate potential.
    J Physiol. 1955 Oct 28;130(1):114-22 PMID: 13278890
  38. The interaction of extracellular H+, Na+, Ca2+ and Sr2+ on the decay of miniature end-plate currents.
    Brain Res. 1982 Jun 10;241(2):285-90 PMID: 6980690
  39. Activation of transmitter release by strontium and calcium ions at the neuromuscular junction.
    J Physiol. 1971 Jul;215(3):709-26 PMID: 4326307
  40. A study of synaptic transmission in the absence of nerve impulses.
    J Physiol. 1967 Sep;192(2):407-36 PMID: 4383089
  41. Properties of a single synapse in the stellate ganglion of squid.
    J Neurophysiol. 1948 Jul;11(4):343-64 PMID: 18872403
  42. The stimulant effect of barium on the release of acetylcholine from the superior cervical ganglion.
    J Physiol. 1961 May;156:515-22 PMID: 13724073
  43. THE EFFECTS OF ALKALINE EARTHS AND OTHER DIVALENT CATIONS ON ADRENAL MEDULLARY SECRETION.
    J Physiol. 1964 Dec;175:231-41 PMID: 14241165
  44. Effects of barium on the potassium conductance of squid axon.
    J Gen Physiol. 1980 Jun;75(6):727-50 PMID: 6248618
  45. Mechanism of activation of a cyclic adenosine 3':5'-monophosphate phosphodiesterase from bovine heart by calcium ions. Identification of the protein activator as a Ca2+ binding protein.
    J Biol Chem. 1973 Sep 10;248(17):5950-5 PMID: 4353626
  46. On the elementary conductance event produced by L-glutamate and quanta of the natural transmitter at the neuromuscular junctions of Maia squinado.
    J Physiol. 1976 Jun;258(1):205-25 PMID: 181565
  47. Tetrodotoxin-resistant electric activity in presynaptic terminals.
    J Physiol. 1969 Aug;203(2):459-87 PMID: 4307710
  48. Facilitation of transmitter release at squid synapses.
    J Gen Physiol. 1978 Oct;72(4):471-86 PMID: 31411
  49. Can barium support the release of acetylcholine by nerve impulses?
    Br J Pharmacol. 1977 Jan;59(1):215-7 PMID: 837001
  50. Effects of divalent cations on toad end-plate channels.
    J Membr Biol. 1982;64(1-2):55-66 PMID: 6276548
  51. Calcium channels in excitable cell membranes.
    Annu Rev Physiol. 1983;45:341-58 PMID: 6303205
  52. Changes in miniature endplate potential frequency during repetitive nerve stimulation in the presence of Ca2+, Ba2+, and Sr2+ at the frog neuromuscular junction.
    J Gen Physiol. 1981 May;77(5):503-29 PMID: 6262429
  53. Calcium ion and muscle contraction.
    Prog Biophys Mol Biol. 1968;18:123-83 PMID: 4894870
  54. The action of extracellular cations on the release of the sympathetic transmitter from peripheral nerves.
    J Physiol. 1967 Mar;189(1):85-99 PMID: 16992247
  55. Effects of [Ca2+] and [Mg2+] on the decay of miniature endplate currents.
    Nature. 1978 Jan 5;271(5640):77-9 PMID: 305002
  56. Stimulus-secretion coupling: the concept and clues from chromaffin and other cells.
    Br J Pharmacol. 1968 Nov;34(3):451-74 PMID: 4882190
  57. Leakage current rectification in the squid giant axon.
    Nature. 1961 Jun 3;190:883-5 PMID: 14448232
  58. Reversal of current through calcium channels in dialysed single heart cells.
    Nature. 1982 Jun 10;297(5866):498-501 PMID: 6283359
  59. Presynaptic currents in mouse motor endings.
    J Physiol. 1982 Dec;333:619-36 PMID: 6304288
  60. Interacting effects of temperature and extracellular calcium on the spontaneous release of transmitter at the frog neuromuscular junction.
    J Physiol. 1977 Jun;268(2):319-33 PMID: 17743
  61. Internal effects of divalent cations on potassium permeability in molluscan neurones.
    J Physiol. 1979 Nov;296:393-410 PMID: 529110
  62. Effects of the ionophore X-537A on acetylcholine release at the frog neuromuscular junction.
    J Physiol. 1976 Jul;259(1):177-98 PMID: 182959
  63. Effect of measured calcium chloride injections on the membrane potential and internal pH of snail neurones.
    J Physiol. 1980 Jan;298:111-29 PMID: 6153718
  64. Metabolism and transport of strontium in giant axons of Loligo.
    J Physiol. 1982 Sep;330:373-92 PMID: 7175747
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1984.sp015020
Published
1984-01-00
Pages
257-71
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1199497
Subset
IM
Grants
NCRR NIH HHS · 5-S07 RR07009-16 · United States
NINDS NIH HHS · NS08364 · United States
External Links
PubMed source DOI: 10.1113/jphysiol.1984.sp015020 PMC Full Text View journal details
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