Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I.

Nucleic acids research ·Vol. 8 ·No. 13 ·1980-07-11 ·Pages 3055-64

Fagan JB, Pastan I, de Crombrugghe B

Abstract

Two cloned cDNAs derived from the mRNA for cell fibronectin have been sequenced, providing evidence that transcription with AMV reverse transcriptase or Escherichia coli DNA polymerase I may not always result in double stranded cDNA that is exactly homologous with its mRNA template. Instead, the sequences of these cloned cDNAs are consistent with the duplication and rearrangement of sequences during synthesis of double stranded cDNA.

MeSH Terms

Animals Avian Leukosis Virus/enzymology Avian Myeloblastosis Virus/enzymology Base Sequence Cell Line Chick Embryo Cloning, Molecular DNA Polymerase I/metabolism DNA Replication DNA Restriction Enzymes DNA, Recombinant/metabolism DNA-Directed DNA Polymerase/metabolism Escherichia coli/enzymology Fibronectins/biosynthesis Nucleic Acid Hybridization Plasmids RNA, Messenger/metabolism RNA-Directed DNA Polymerase/metabolism

Chemicals

DNA, Recombinant Fibronectins RNA, Messenger RNA-Directed DNA Polymerase DNA Polymerase I DNA-Directed DNA Polymerase DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Fagan J B

Pastan I

de Crombrugghe B

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.13.3055

Published

1980-07-11

Pages

3055-64

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324144

Subset

Databases

GENBANK

V00403, V00404, V00405

External Links

PubMed source DOI: 10.1093/nar/8.13.3055 PMC Full Text View journal details

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Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I.

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