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PMID: 6163136 Published · ppublish English Journal Article

The absence of introns within a human fibroblast interferon gene.

Nucleic acids research ·Vol. 9 ·No. 2 ·1981-01-24 ·Pages 247-66

Houghton M, Jackson IJ, Porter AG, Doel SM, Catlin GH, Barber C, Carey NH

Abstract

Experiments in which immobilised restriction fragments of genomic DNA were hybridised with a cloned human fibroblast interferon cDNA indicate that the homologous chromosomal genes exist in only one basic arrangement. This is in marked contrast to recent studies by Nagata et al. (1) showing that there are at least eight gene arrangements for human leukocyte interferon. Having isolated a chromosomal human fibroblast interferon gene from a gene bank, we conclude from nucleotide sequencing studies that there is a complete absence of introns within the RNA-coding region. In view of a similar observation recently made for a human leukocyte interferon gene (1), it would appear as if interferon genes in general are unlike the vast majority of eukaryote genes in this respect.

MeSH Terms
Base Sequence Chromosomes, Human/metabolism Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Fibroblasts/metabolism Genes Humans Interferons/genetics Leukocytes/metabolism Nucleic Acid Hybridization Protein Biosynthesis
Chemicals
DNA, Recombinant Interferons DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Houghton M
Jackson I J
Porter A G
Doel S M
Catlin G H
Barber C
Carey N H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.2.247
Published
1981-01-24
Pages
247-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326690
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.2.247 PMC Full Text View journal details
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