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PMID: 6174942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.

Nucleic acids research ·Vol. 10 ·No. 3 ·1982-02-11 ·Pages 967-77

Ohno S, Taniguchi T

Abstract

A hybrid plasmid was constructed from pSV2-Ecogpt (Mulligan and Berg, 1981) and the cloned 1.8 kilobase chromosomal DNA segment which contains the human interferon (HIFN-beta 1) gene and its flanking sequences. Cultured mouse FM3A cells were transfected by the hybrid plasmid and several Ecogpt+ clones were selected. Southern blot analysis of the DNA from these mouse cell clones showed that two of them contained a few copies of the 1.8 kilobase human DNA. Synthesis of mRNA characteristic of the HIFN-beta 1 mRNA increased as measured by RNA blot analysis when those clones were treated with Newcastle disease virus or with poly(I):poly(C). In addition, the induced mouse cell clones gave rise to the synthesis of human IFN-beta 1. These results suggest the presence of a unique nucleotide sequence in the 1.8 kilobase DNA which is sufficient for the inducer-responsive expression of the human IFN-beta 1 gene.

MeSH Terms
Animals Base Sequence Cell Line Cloning, Molecular DNA Restriction Enzymes Female Genes Humans Interferons/genetics Mammary Neoplasms, Experimental/immunology Mice Newcastle disease virus/immunology Nucleic Acid Hybridization Plasmids Poly I-C/pharmacology Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger Interferons DNA Restriction Enzymes Poly I-C
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohno S
Taniguchi T
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.3.967
Published
1982-02-11
Pages
967-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326214
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.3.967 PMC Full Text View journal details
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