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PMID: 6184719 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Selective amplification of polymorphic dihydrofolate reductase gene loci in Chinese hamster lung cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 22 ·1982-11-00 ·Pages 6961-5

Lewis JA, Davide JP, Melera PW

Abstract

A series of antifolate-resistant Chinese hamster lung sublines that overproduce either a Mr 20,000 or a Mr 21,000 class of dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3), known to contain amplified DHFR genes, has been analyzed by DNA and RNA transfer techniques. The results suggest that the Mr 20,000 and Mr 21,000 DHFRs are encoded by at least two polymorphic DHFR genes, both of which are expressed in drug-sensitive parental cells. In drug-resistant sublines only one of the two DHFR gene types is amplified, thus accounting for the overproduction of one or the other molecular weight class of DHFR. In addition to the known differences between the DHFRs whose overproduction they direct, these allelic genes differ in restriction endonuclease profiles and in the relative abundances of their multiple mRNA transcripts.

MeSH Terms
Animals Cell Line Cricetinae Cricetulus Drug Resistance Folic Acid Antagonists/pharmacology Gene Amplification Genes Lung Poly A/genetics Polymorphism, Genetic RNA/genetics RNA, Messenger Tetrahydrofolate Dehydrogenase/genetics
Chemicals
Folic Acid Antagonists RNA, Messenger Poly A RNA Tetrahydrofolate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lewis J A
Davide J P
Melera P W
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.22.6961
Published
1982-11-00
Pages
6961-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347254
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.22.6961 PMC Full Text View journal details
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