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PMID: 6258555 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Repetitive DNA sequences in methotrexate- and methasquin-sensitive and -resistant Chinese hamster cell lines.

Biochemical genetics ·Vol. 18 ·No. 7-8 ·1980-08-00 ·Pages 655-67

Wolgemuth DJ, Biedler JL, Melera PW

Abstract

DNA purified from a Chinese hamster cell line of lung fibroblast origin (DC83F) was analyzed by density gradient centrifugation and by gel electrophoresis after restriction endonuclease digestion in order to fractionate discrete repetitive fractions within the total DNA. No obvious satellite DNAs were resolved using the CsCl or Ag-Cs2SO4 density gradient conditions described herein. However, analysis of the digestion products of a battery of restriction endonucleases indicated that three of these enzymes, EcoR1, HaeIII, and XhoI, yielded discrete fragments which could be visualized with EtBr staining or identified by scintillation counting of [3H]DNA. DNAs from several highly (greater than or equal to hundredfold increased resistance) antifolate-resistant sublines of DC-3F, characterized by a large homogeneously staining region (HSR) in the chromosome complement, were examined with both techniques and compared to the parental, antifolate-sensitive cell line DNA. The density gradient profiles and electrophoretic patterns of restriction endonuclease digests were identical among all the cell lines examined and were indistinguishable from those of the parental DC-3F DNA.

MeSH Terms
Animals Aspartic Acid/analogs & derivatives,pharmacology Base Sequence Cell Line Centrifugation, Density Gradient Cricetinae Cricetulus DNA/analysis DNA Replication DNA Restriction Enzymes/metabolism Drug Resistance Electrophoresis, Polyacrylamide Gel Lung Methotrexate/pharmacology Phenotype
Chemicals
methasquin Aspartic Acid DNA DNA Restriction Enzymes Methotrexate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wolgemuth D J
Biedler J L
Melera P W
References (37)
37 references, click to expand
  1. Repeating restriction fragments of human DNA.
    Nucleic Acids Res. 1976 Nov;3(11):3063-76 PMID: 794832
  2. Distribution of constitutive heterochromatin in mamallian chromosomes.
    Chromosoma. 1971;34(3):243-53 PMID: 5112131
  3. Buoyant densities of DNA of mammals.
    Biochem Genet. 1970 Jun;4(3):367-76 PMID: 4991030
  4. Drug response, dihydrofolate reductase, and cytogenetics of amethopterin-resistant Chinese hamster cells in vitro.
    Cancer Res. 1972 Jan;32(1):153-61 PMID: 5007683
  5. Intragenic DNA spacers interrupt the ovalbumin gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1299-303 PMID: 274719
  6. An analysis of eukaryotic genomes by density gradient centrifugation.
    J Mol Biol. 1976 Nov;108(1):219-35 PMID: 826643
  7. Equilibrium sedimentation in density gradients of DNA preparations from animal tissues.
    J Mol Biol. 1961 Dec;3:711-6 PMID: 14456492
  8. Biochemical and karyological properties of cells resistant to the quinazoline antifolate, methasquin.
    Eur J Cancer. 1978 Jan;14(1):41-9 PMID: 564273
  9. Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5553-6 PMID: 281704
  10. Location of satellite and homogeneous DNA sequences on human chromosomes.
    Nat New Biol. 1971 Oct 27;233(43):268-71 PMID: 4107945
  11. Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
    Proc Natl Acad Sci U S A. 1968 Jun;60(2):553-60 PMID: 5248812
  12. A satellite DNA isolated from human tissues.
    J Mol Biol. 1967 Feb 14;23(3):619-22 PMID: 6032194
  13. The chromosomal location of human satellite DNA 3.
    Chromosoma. 1973 Jul 18;42(4):445-51 PMID: 4730561
  14. Repeated sequence specific to human males.
    Nature. 1976 Jul 15;262(5565):182-6 PMID: 819844
  15. Marked increase in ribosomal RNA gene multiplicity in a rat hepatoma cell line.
    Chromosoma. 1979 Feb 21;71(2):183-95 PMID: 428274
  16. Antifolate-resistant chinese hamster cells. Evidence from independently derived sublines for the overproduction of two dihydrofolate reductases encoded by different mRNAs.
    J Biol Chem. 1980 Jan 25;255(2):319-22 PMID: 7356612
  17. Localisation of a male-specific DNA fragment to a sub-region of the human Y chromosome.
    Nature. 1978 Mar 23;272(5651):324-8 PMID: 76288
  18. Fractions of HeLa DNA differing in their content of guanine+cytosine.
    J Mol Biol. 1969 Dec 14;46(2):305-12 PMID: 5360041
  19. Chromosomal locations of satellite DNA in the MSWBS ascites tumor cell line.
    J Natl Cancer Inst. 1977 Apr;58(4):1151-3 PMID: 845985
  20. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
    Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
  21. Isolation of the complementary strands of a human satellite DNA.
    J Mol Biol. 1968 Apr 14;33(1):331-5 PMID: 5646653
  22. The distribution of moderately repeated DNA sequences among Chinese hamster chromosomes.
    Exp Cell Res. 1978 Mar 1;112(1):155-65 PMID: 631209
  23. Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.
    J Biol Chem. 1978 Mar 10;253(5):1357-70 PMID: 627542
  24. Metaphase chromosome anomaly: association with drug resistance and cell-specific products.
    Science. 1976 Jan 16;191(4223):185-7 PMID: 942798
  25. Search for DNA interchange corresponding to sister chromatid exchanges in Chinese hamster ovary cells.
    Nucleic Acids Res. 1978 Nov;5(11):4087-104 PMID: 724509
  26. The location of four human satellite DNAs on human chromosomes.
    Exp Cell Res. 1975 Apr;92(1):148-58 PMID: 48464
  27. Chromosomal localization of complex and simple repeated human DNAs.
    Chromosoma. 1978 Mar 22;66(1):23-32 PMID: 639625
  28. Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.
    Chromosoma. 1977 Jun 23;61(4):335-44 PMID: 880841
  29. An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl)dioxane density gradient.
    Eur J Biochem. 1977 Jun 1;76(1):13-9 PMID: 880946
  30. Fractions of Chinese hamster DNA differing in their content of guanine+cytosine and evidence for the presence of single-stranded DNA.
    Biochim Biophys Acta. 1972 Jul 20;272(3):350-60 PMID: 4340342
  31. A simplified method for preparation of mouse satellite DNA.
    Anal Biochem. 1977 Apr;78(2):561-8 PMID: 557911
  32. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.
    Chromosoma. 1973;41(2):129-44 PMID: 4120885
  33. Complex and simple sequences in human repeated DNAs.
    Chromosoma. 1978 Mar 22;66(1):1-21 PMID: 639624
  34. An analysis of the bovine genome by Cs2SO4-Ag density gradient centrifugation.
    J Mol Biol. 1973 Oct 15;80(1):177-97 PMID: 4798988
  35. Identification and separation of components of calf thymus DNA using a CsC1-netropsin density gradient.
    Nucleic Acids Res. 1975 Mar;2(3):431-46 PMID: 805418
  36. Amplification of dihydrofolate reductase genes in methotrexate-resistant cultured mouse cells.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:649-57 PMID: 277312
  37. The effect of silver ion binding and pH on the buoyant density of DNA and its use in fractionating heterogeneous DNA.
    Biochim Biophys Acta. 1976 Apr 2;425(4):451-62 PMID: 4104
Article Info
Journal
Biochemical genetics
Abbr.
Biochem Genet
ISSN
0006-2928
DOI
10.1007/BF00484583
Published
1980-08-00
Pages
655-67
Language
English
Region
United States
NLM ID
0126611
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00484583 View journal details
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