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PMID: 6185281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Visualization of Ag-NOR proteins on nucleolar transcriptional units in molecular spreads.

Chromosoma ·Vol. 86 ·No. 5 ·1982-00-00 ·Pages 661-72

Angelier N, Hernandez-Verdun D, Bouteille M

Abstract

Application of NOR silver staining to Pleurodeles oocyte nuclei showed selective silver deposits on the fibrillar part of the nucleoli in situ. This staining was adapted to nucleoli spread on grids, by a procedure which allowed the spreading of transcription units from both nucleoli and lampbrush chromosomes on the same grids. This permitted localization of the predominant nucleolar Ag-stained proteins on the nucleolar transcriptional units and not on the lampbrush chromosomes. These proteins were found exclusively on the transcribed part of the nucleolar genes and were not seen in apparently untranscribed spacer regions. The proteins seemed preferentially located on the DNP axis rather than on the RNP fibrils.

MeSH Terms
Animals Cell Nucleolus/metabolism Female Oocytes/cytology,metabolism Pleurodeles Silver Proteins Staining and Labeling Transcription, Genetic
Chemicals
Silver Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Angelier N
Hernandez-Verdun D
Bouteille M
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34 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00285609
Published
1982-00-00
Pages
661-72
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00285609 View journal details
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