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PMID: 6190129 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and analysis of cDNA sequences coding for two 16 kilodalton heat shock proteins (hsps) in Caenorhabditis elegans: homology with the small hsps of Drosophila.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3187-205

Russnak RH, Jones D, Candido EP

Abstract

The nucleotide sequences of two different cDNAs, CEHS48 and CEHS41, coding for the 16,000 dalton heat shock proteins (hsps) of Caenorhabditis elegans have been determined. CEHS48 codes for a polypeptide of 135 amino acids, approximately 15 fewer than the complete protein while CEHS41 is missing approximately 46 amino acids. From nucleotide 113 to the TAA termination signal the extent of homology between the sequences is 91%. Toward the 5' ends, the homology drops to 20% and results in completely divergent amino acid sequences. The 3' noncoding regions are only 30% homologous. Only CEHS48 contains a poly(A) signal and a poly(A) tail, suggesting that CEHS41 has an incomplete 3' end. The region from amino acid 43 to amino acid 115 shows extensive homology with corresponding regions in the four small hsps of Drosophila melanogaster and in mammalian alpha-crystallin. Two-dimensional gel analysis of in vitro synthesized hsp16 reveals the existence of five distinct components of identical molecular weights, but with different isoelectric points.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Caenorhabditis/genetics Cloning, Molecular DNA/analysis Drosophila melanogaster/genetics Heat-Shock Proteins Hot Temperature Molecular Weight Nucleic Acid Hybridization Protein Biosynthesis Proteins/genetics RNA/genetics RNA, Messenger/genetics Species Specificity Transcription, Genetic
Chemicals
Heat-Shock Proteins Proteins RNA, Messenger RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Russnak R H
Jones D
Candido E P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.3187
Published
1983-05-25
Pages
3187-205
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325957
Subset
IM
Databases
GENBANK
K01863, K01864
External Links
PubMed source DOI: 10.1093/nar/11.10.3187 PMC Full Text View journal details
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