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PMID: 6201827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Use of complementary DNA oligomers to probe trp leader transcript secondary structures involved in transcription pausing and termination.

Nucleic acids research ·Vol. 12 ·No. 7 ·1984-04-11 ·Pages 3295-302

Fisher R, Yanofsky C

Abstract

DNA oligomers were synthesized that are perfectly complementary to different segments of the tryptophan (trp) operon leader transcript. These 15 nucleotide long oligomers were used as probes of the involvement of transcript secondary structures in two processes: transcription pausing at the pause site located near base pair 90 in the leader region, and transcription termination at the attenuator. The 15-mers were complementary to the four segments of the trp leader transcript which have been shown to form the alternative secondary structures that are believed to be responsible for pausing, termination, and antitermination. Oligomers complementary to RNA segments 1 and 3 relieved termination while the 15-mer complementary to RNA segment 1 relieved pausing. 15-mers complementary to segment 2 had no effect on pausing and the oligomer complementary to segment 4 had virtually no effect on termination.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Half-Life Kinetics Nucleic Acid Conformation Oligodeoxyribonucleotides/metabolism Oligonucleotides/metabolism Operon RNA, Bacterial/genetics Transcription, Genetic Tryptophan/metabolism
Chemicals
DNA, Bacterial Oligodeoxyribonucleotides Oligonucleotides RNA, Bacterial Tryptophan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fisher R
Yanofsky C
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.7.3295
Published
1984-04-11
Pages
3295-302
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318746
Subset
IM
Grants
NIGMS NIH HHS · GM-09738 · United States
External Links
PubMed source DOI: 10.1093/nar/12.7.3295 PMC Full Text View journal details
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