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PMID: 6202510 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibody against the carboxy terminal peptide of pp60src of Rous sarcoma virus reacts with native pp60src.

The EMBO journal ·Vol. 1 ·No. 12 ·1982-00-00 ·Pages 1479-85

Tamura T, Bauer H

Abstract

A monoclonal mouse antibody has been prepared against a synthetic peptide corresponding to the six carboxy-terminal amino acids (C' peptide) of the src gene product pp60v -src of Rous sarcoma virus (RSV). The antibody was able to precipitate pp60v -src and to bind pp60v -src kinase activity in a competition test, indicating that this peptide can serve as an antibody-binding site (epitope). Furthermore, the finding that three out of 28 pp60src-specific tumor-bearing rabbit (TBR) sera contained antibody against the C' peptide argues for an in vivo role for the carboxy terminus of pp60src. C' peptide-specific IgG was purified from one TBR serum using affinity chromatography, and was shown to precipitate significant amounts of pp60src, and bind most of the pp60src kinase activity from SRA, PrA, and B77-C strains of avian sarcoma virus (ASV), but not endogenous pp60c -src, a cellular homologue to the viral pp60v -src. Similar results were obtained with IgG isolated from a C' peptide immune rabbit serum. None of the three C' peptide-specific IgGs could serve as a phosphate acceptor in an immune complex protein kinase reaction.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Avian Sarcoma Viruses/metabolism Cell Transformation, Neoplastic Cells, Cultured Chick Embryo Epitopes/analysis Hybridomas/immunology Mice Oncogene Protein pp60(v-src) Peptide Fragments/analysis Plasmacytoma/immunology Protein Kinases/metabolism Viral Proteins/metabolism
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Epitopes Peptide Fragments Viral Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tamura T
Bauer H
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1479-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553239
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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