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PMID: 6210879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unrearranged immunoglobulin variable region genes have a functional promoter.

Nucleic acids research ·Vol. 10 ·No. 6 ·1982-03-25 ·Pages 1841-56

Bentley DL, Farrell PJ, Rabbitts TH

Abstract

We have tested whether immunoglobulin light chain variable region genes are capable of directing initiation of transcription without undergoing the DNA rearrangement which creates a complete immunoglobulin gene. Two human Vk genes specifically initiated transcription in vitro at a site which is approximately 30 bases downstream of a TATA box and 20 bases upstream of the initiation codon. A Vk pseudogene which lacks a TATA box at an homologous position was not transcribed to a detectable extent in the in vitro system. One of the Vk genes was injected into Xenopus oocytes and it initiated transcription at precisely the same position as in the HeLa cell extract. It is suggested that the promoter which we have identified upstream of unrearranged Vk genes operates in lymphocytes after V-J joining has occurred to initiate transcription of light chain messenger RNA.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Binding Sites, Antibody/genetics Cloning, Molecular Female Fetus Genes HeLa Cells/metabolism Humans Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region/genetics Liver Nucleic Acid Hybridization Oocytes/metabolism Operon Plasmids Pregnancy Templates, Genetic Transcription, Genetic
Chemicals
Immunoglobulin Light Chains Immunoglobulin Variable Region
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bentley D L
Farrell P J
Rabbitts T H
References (41)
41 references, click to expand
  1. Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3628-32 PMID: 824647
  2. Purified DNAs are transcribed after microinjection into Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1502-6 PMID: 193103
  3. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  4. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  5. Evidence for noncontiguous variable and constant region genes in both germ line and myeloma DNA.
    Cell. 1978 Feb;13(2):319-27 PMID: 414842
  6. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  7. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  8. Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
    Dev Biol. 1978 May;64(1):178-88 PMID: 207607
  9. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  10. Cloning of nematode tRNA genes and their expression in the frog oocyte.
    Nucleic Acids Res. 1978 Dec;5(12):4593-611 PMID: 370775
  11. Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.
    Nucleic Acids Res. 1979 Jan;6(1):195-203 PMID: 218170
  12. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  13. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  14. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
    Cell. 1979 Oct;18(2):469-84 PMID: 498279
  15. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
    Cell. 1979 Oct;18(2):485-99 PMID: 498280
  16. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  17. Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes.
    J Biol Chem. 1980 Apr 25;255(8):3691-4 PMID: 6767723
  18. Synthetic adaptors for cloning DNA.
    Methods Enzymol. 1979;68:98-109 PMID: 232227
  19. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  20. Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei.
    Nature. 1980 Jun 26;285(5767):628-34 PMID: 7190224
  21. Structure and in vitro transcription of human globin genes.
    Science. 1980 Sep 19;209(4463):1329-36 PMID: 6158093
  22. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  23. Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
    Nature. 1980 Nov 6;288(5786):100-2 PMID: 7432506
  24. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  25. The primary structure of the human epsilon-globin gene.
    Cell. 1980 Oct;21(3):621-6 PMID: 6254663
  26. Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10 PMID: 6255460
  27. Transcription units of chicken ovalbumin gene observed after injection of cloned complete genes into Xenopus oocyte nuclei.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5984-8 PMID: 6934527
  28. Human immunoglobulin variable region genes--DNA sequences of two V kappa genes and a pseudogene.
    Nature. 1980 Dec 25;288(5792):730-3 PMID: 6779204
  29. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  30. The SV40 early region TATA box is required for accurate in vitro initiation of transcription.
    Nature. 1981 Mar 26;290(5804):310-5 PMID: 6259539
  31. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  32. Specific 5' flanking sequences are required for faithful initiation of in vitro transcription of the ovalbumin gene.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):879-83 PMID: 6262780
  33. Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
    Cell. 1981 May;24(2):333-44 PMID: 7237551
  34. Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.
    Nature. 1981 Jun 25;291(5817):668-70 PMID: 6264318
  35. Human V kappa immunoglobulin gene number: implications for the origin of antibody diversity.
    Cell. 1981 Jun;24(3):613-23 PMID: 6265099
  36. Localization of DNA sequences necessary for transcription of the rabbit beta-globin gene in vitro.
    Cell. 1981 Jul;25(1):215-26 PMID: 7273136
  37. Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
    Cell. 1981 Aug;25(2):385-98 PMID: 6269744
  38. Promotion of specific in vitro transcription by excised "TATA" box sequences inserted in a foreign nucleotide environment.
    Nucleic Acids Res. 1981 Aug 25;9(16):3941-58 PMID: 7301576
  39. Promoters and heterogeneous 5' termini of the messenger RNAs of adenovirus serotype 2.
    J Mol Biol. 1981 Jun 25;149(2):189-221 PMID: 6927849
  40. Transcriptional regulation of immunoglobulin V genes.
    Nucleic Acids Res. 1981 Dec 21;9(24):6855-67 PMID: 6801628
  41. Transcription of the unrearranged mouse C kappa locus: sequence of the initiation region and comparison of activity with a rearranged V kappa-C kappa gene.
    Cell. 1981 Dec;27(3 Pt 2):593-602 PMID: 6101210
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.6.1841
Published
1982-03-25
Pages
1841-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320575
Subset
IM
Databases
GENBANK
J00247, J00250, J00251
External Links
PubMed source DOI: 10.1093/nar/10.6.1841 PMC Full Text View journal details
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