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PMID: 6224774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Temperature-inducible amber suppressor: construction of plasmids containing the Escherichia coli serU- (supD-) gene under control of the bacteriophage lambda pL promoter.

Journal of bacteriology ·Vol. 155 ·No. 3 ·1983-09-00 ·Pages 1417-25

Steege DA, Horabin JI

Abstract

An Escherichia coli DNA fragment containing the structural gene serU132 for the nonsense suppressor tRNASer2am was identified and purified by being cloned into a plasmid vector. Information obtained from DNA sequence analysis was used to select a serU132 fragment for insertion downstream from the bacteriophage lambda pL promoter in two pBR322-lambda derivatives. In nonsense mutant strains bearing the resulting serU132 hybrid plasmids, the presence of the lambda cI857 repressor gene carried on the same plasmid or in a prophage genome permits thermal regulation of suppressor synthesis.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Escherichia coli/genetics Mutation Operon Plasmids RNA, Transfer, Amino Acyl/genetics Suppression, Genetic Temperature
Chemicals
RNA, Transfer, Amino Acyl
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Steege D A
Horabin J I
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.3.1417-1425.1983
Published
1983-09-00
Pages
1417-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217842
Subset
IM
Grants
NCI NIH HHS · CA-14236 · United States
Databases
GENBANK
K01667
External Links
PubMed source DOI: 10.1128/jb.155.3.1417-1425.1983 PMC Full Text View journal details
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