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PMID: 6227793 Published · ppublish English Comparative Study Journal Article

Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 192 ·No. 1-2 ·1983-00-00 ·Pages 10-4

Yamagata H, Taguchi N, Daishima K, Mizushima S

Abstract

A mutation (lspA, prolipoprotein signal peptidase) rendering the prolipoprotein signal peptidase temperature-sensitive in Escherichia coli has been analyzed. The mutation was mapped in the dnaJ-rpsT-ileS-dapB region by interrupted mating with various Hfr strains and P1 phage transduction. lambda transducing phage lambda ddapB2 that carries the rpsT-ileS-dapB region was shown to complement the lspA mutation. Plasmid pLC3-13 which had been isolated from Clarke and Carbon's collection as a plasmid carrying the lspA locus was shown to carry the dnaJ and rpsT loci. Complementation analysis with plasmids carrying various DNA fragments derived from pLC3-13 showed that the lspA locus is between the rpsT and ileS loci. The wildtype allele was dominant over the lspA allele.

MeSH Terms
Bacteriophage lambda/genetics Chromosome Mapping Endopeptidases/genetics Escherichia coli/enzymology,genetics Genes, Bacterial Genetic Complementation Test Membrane Proteins Mutation Plasmids Serine Endopeptidases
Chemicals
Membrane Proteins Endopeptidases Serine Endopeptidases type I signal peptidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamagata H
Taguchi N
Daishima K
Mizushima S
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33 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327640
Published
1983-00-00
Pages
10-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327640
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