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PMID: 6235918 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Patterns of lambda Int recognition in the regions of strand exchange.

Cell ·Vol. 33 ·No. 1 ·1983-05-00 ·Pages 261-72

Ross W, Landy A

Abstract

Int protein has two classes of binding sites within the phage att site: the arm-type recognition sequences are found in three specific sites that are distant from the region of strand exchange; the junction-type recognition sequences occur as inverted pairs around the crossover region in both attP and attB. During recombination between attP and attB each of the four DNA strands is cut at a homologous position within each of the junction-type Int binding sites. In all four junction-type sites Int protein interacts primarily with the same face of the DNA helix, as determined by those purine nitrogens that are protected against methylation by dimethylsulfate. Efficient secondary attachment sites for lambda contain sequences with partial homology to the junction-type binding sites. In addition, the sequence between, but not part of, the two junction-type sites (the overlap region) is strongly conserved in secondary att sites. Thus, in the vicinity of strand exchange, attP and a recombining partner, such as attB, are very similar; each comprises two junction-type Int recognition sites and an overlap (crossover) region.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Binding Sites DNA, Viral/genetics Genes, Viral Lysogeny Methylation Recombination, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ross W
Landy A
References (38)
38 references, click to expand
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(83)90355-0
Published
1983-05-00
Pages
261-72
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2211524
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007601 · United States
NIGMS NIH HHS · 5 T32 GM 07601 · United States
NIGMS NIH HHS · R01 GM033928 · United States
NIAID NIH HHS · AI 13544 · United States
NIGMS NIH HHS · R01 GM062723 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(83)90355-0 PMC Full Text View journal details
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