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PMID: 6245216 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural and functional membrane polarity in cultured monolayers of MDCK cells.

The Journal of membrane biology ·Vol. 52 ·No. 2 ·1980-00-00 ·Pages 147-59

Cereijido M, Ehrenfeld J, Meza I, Martínez-Palomo A

Abstract

MDCK cells form monolayers which have many of the properties usually found in transporting epithelia. The present article is devoted to the study of the structural and functional polarization of MDCK cells, which is one of the central features of transporting epithelia. The results show: (i) that MDCK monolayers transport 2.6 mumol hr-1 cm-2 of sodium in the apical to basolateral direction; (ii) the passive flux of this ion is relatively large (20.3 mole hr-1 cm-2), which is a characteristic of leaky epithelia; (iii) a large fraction of the penetration of sodium into the cells proceeds through an amiloride-sensitive channel, and the exit is operated mainly by a ouabain-sensitive pump; (iv) the net transport of sodium from the apical to the basolateral side agrees with the asymmetric labeling of the pumps with 3H-ouabain; (v) this asymmetric labeling agrees, in turn, with a higher concentration of intramembrane particles (IMPs) in freeze-fracture replicas of the basolateral side of the plasma membrane; (vi) the structural polarization of confluent MDCK cells is also revealed by the location of microvilli, occluding junctions, and pinocytotic vesicles; and (vii) the presence of a continuous ring formed by actin microfilaments visualized by immunofluorescence under the lateral aspect of the plasma membrane that may be related to the distribution of the occluding junctions, which act as barriers separating apical from basolateral membrane components.

MeSH Terms
Amiloride/pharmacology Animals Biological Transport Cell Membrane/physiology,ultrastructure Cells, Cultured Cytoskeleton/ultrastructure Dogs Female Fluorescent Antibody Technique Freeze Fracturing Ion Channels Kidney/cytology Kinetics Microscopy, Electron Microtubules/ultrastructure Ouabain/pharmacology Sodium/metabolism
Chemicals
Ion Channels Ouabain Amiloride Sodium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cereijido M
Ehrenfeld J
Meza I
Martínez-Palomo A
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869120
Published
1980-00-00
Pages
147-59
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01869120 View journal details
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