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PMID: 6248855 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of the closely linked adult chicken alpha-globin genes in recombinant DNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2596-600

Engel JD, Dodgson JB

Abstract

Recombinant bacteriophage (from a library of chicken chromosomal DNA fragments inserted into lambda Charon 4A) have been isolated which contain the coding information for both of the adult chicken alpha-globin genes, alpha A and alpha D. One of these recombinant phage also contains an as yet unidentified embryonic alpha-like globin gene sequence. The two adult genes are encoded on the same DNA strand and are separated by approximately 2.4 kilobase pairs, with the arrangement of the genes relative to the direction of transcription being 5'-alpha D-alpha A-3'. Electron microscopic R-loop visualization experiments demonstrate that both alpha-globin genes contain two intervening sequences of similar size in a manner analogous to the structure observed in the mouse alpha-globin gene. The linkage of the two highly divergent chicken adult alpha-globin genes further underscores the principle that chromosomal clustering of families of developmentally related genes may be a general phenomenon in higher eukaryotic gene sequence arrangement.

MeSH Terms
Animals Base Sequence Chickens/genetics Chromosome Mapping Codon DNA Restriction Enzymes DNA, Recombinant Genes Genetic Linkage Globins/genetics Molecular Weight Transcription, Genetic
Chemicals
Codon DNA, Recombinant Globins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Engel J D
Dodgson J B
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2596
Published
1980-05-00
Pages
2596-600
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349449
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.2596 PMC Full Text View journal details
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