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PMID: 6259130 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Homology between Escherichia coli plasmids ColE1 and p15A.

Journal of bacteriology ·Vol. 145 ·No. 3 ·1981-03-00 ·Pages 1305-9

Bird RE

Abstract

The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids contained the entire region of ColE1 required for its replication as well as an additional 400 base pairs downstream from the origin of replication. This region on p15A, which was 980 +/- 43 base pairs, started at 0.1 of the molecular length from one end formed by cleavage with the restriction endonuclease BglI and extended to 0.54 of the molecular length from the same end. Restriction cleavage maps for the enzymes BglI, HpaI, HaeII, HaeIII, and HincII are also presented.

MeSH Terms
Base Sequence DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Plasmids
Chemicals
Nucleic Acid Heteroduplexes DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GCCNNNNNGGC-specific type II deoxyribonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bird R E
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.3.1305-1309.1981
Published
1981-03-00
Pages
1305-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217133
Subset
IM
Grants
NIGMS NIH HHS · GM 25526 · United States
NCRR NIH HHS · S07-RR07089-14 · United States
External Links
PubMed source DOI: 10.1128/jb.145.3.1305-1309.1981 PMC Full Text View journal details
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