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PMID: 6267420 Published · ppublish English Journal Article

Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance.

Molecular & general genetics : MGG ·Vol. 181 ·No. 4 ·1981-00-00 ·Pages 464-9

Yamamoto T, Tanaka M, Baba R, Yamagishi S

Abstract

A map of cleavage sites for restriction endonuclease EcoRI, BamHI, HindIII, and SalI on Tn2603, a transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury, was constructed by an analysis of restriction cleavage patterns of plasmid pMK1.::Tn2603 and its deletion derivative. By cloning the fragments generated from pMK1.::Tn2603 with these restriction endonucleases to a pACYC184 plasmid vehicle, the regions necessary for expression of resistance were located on the restriction cleavage map of Tn2603. Ampicillin, streptomycin, and sulfonamide-resistance genes were mapped in a cluster on the region between the center and the right and the mercury-resistance gene was located to the left of the map. The final functional map of Tn2603 was compared with those of Tn4 and Tn21 and the evolutional relationships between them were discussed.

MeSH Terms
Chromosome Mapping DNA Restriction Enzymes/metabolism DNA Transposable Elements DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/drug effects,genetics Mercury/pharmacology Penicillin Resistance Plasmids Streptomycin/pharmacology Sulfonamides/pharmacology
Chemicals
DNA Transposable Elements DNA, Bacterial Sulfonamides DNA Restriction Enzymes Mercury Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamamoto T
Tanaka M
Baba R
Yamagishi S
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00428737
Published
1981-00-00
Pages
464-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00428737
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