Home LiteratureArticle Details
PMID: 6274842 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae have retained an intact right part of a Tn3-like transposon.

Journal of bacteriology ·Vol. 149 ·No. 1 ·1982-01-00 ·Pages 136-44

Fayet O, Froment Y, Piffaretti JC

Abstract

In three beta-lactamase-producing strains of Neisseria gonorrhoeae, ampicillin resistance is due to the presence of a 7.4-kilobase plasmid. Heteroduplex analysis has shown that the R plasmid contains a 1.6-kilobase segment homologous to the right part (the region coding for the beta-lactamase) of the Tn3-like transposon Tn2301 the 1.6-kilobase DNA segment is not transposable, but it can give rise to a functional transposon, when linked to the left part of TN2301. This provides strong evidence that the R plasmids of N. gonorrhoeae are deletion derivatives of a plasmid that contained an entire TN3-like transposon.

MeSH Terms
Ampicillin/pharmacology DNA Transposable Elements Neisseria gonorrhoeae/drug effects,enzymology,genetics Nucleic Acid Heteroduplexes Nucleic Acid Hybridization R Factors beta-Lactamases/genetics
Chemicals
DNA Transposable Elements Nucleic Acid Heteroduplexes Ampicillin beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fayet O
Froment Y
Piffaretti J C
References (22)
22 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Phage-lambda-mediated transduction of non-conjugative plasmids is promoted by transposons.
    Gene. 1981 Apr;13(3):319-25 PMID: 6266920
  3. DNA-DNA hybridization on filters at low temperature in the presence of formamide or urea.
    Biochimie. 1971;53(10):1111-4 PMID: 4948624
  4. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae.
    Infect Immun. 1974 Oct;10(4):712-7 PMID: 4214778
  5. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae.
    J Bacteriol. 1975 Jun;122(3):1293-300 PMID: 807554
  6. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  9. Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae.
    J Bacteriol. 1977 Aug;131(2):557-63 PMID: 407214
  10. Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1.
    Mol Gen Genet. 1977 Jun 24;153(3):289-95 PMID: 331072
  11. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  12. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  13. Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function.
    J Bacteriol. 1979 May;138(2):510-8 PMID: 108261
  14. Effect of temperature on translocation frequency of the Tn3 element.
    J Bacteriol. 1979 Aug;139(2):515-9 PMID: 378977
  15. Molecular characterization of a small Haemophilus influenzae plasmid specifying beta-lactamase and its relationship to R factors from Neisseria gonorrhoeae.
    J Gen Microbiol. 1979 Mar;111(1):223-31 PMID: 110907
  16. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  17. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.
    Cell. 1979 Dec;18(4):1153-63 PMID: 391406
  18. A comprehensive molecular map of bacteriophage lambda.
    Gene. 1979 Nov;7(3-4):217-70 PMID: 160360
  19. Recombination between bacteriophage lambda and plasmid pBR322 in Escherichia coli.
    J Bacteriol. 1980 Jun;142(3):992-1003 PMID: 6247334
  20. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  21. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  22. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.149.1.136-144.1982
Published
1982-01-00
Pages
136-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216601
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.149.1.136-144.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2