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PMID: 6276677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Integration specificity of an artificial kanamycin transposon constructed by the in vitro insertion of an internal Tn5 fragment into IS2.

Molecular & general genetics : MGG ·Vol. 183 ·No. 1 ·1981-00-00 ·Pages 45-50

Saint-Girons I, Fritz HJ, Shaw C, Tillmann E, Starlinger P

Abstract

IS2 has been marked genetically by the in vitro insertion into its HindIII site of a 3.3 Kb HindIII fragment of Tn5 conferring resistance to kanamycin. The transposition of the IS2::Km, thus obtained, to lambda has been found and insertion sites were characterised. Each of ten independent IS2::Km insertions were found at the same site at 61.2% of the lambda map, always in the same orientation (orientation II relative to the xis gene). The integration sites of IS2::Km in five of the kanamycin-transducing phages were determined by DNA sequence analysis, and were found to be identical at the nucleotide level. Further transposition of IS2::Km from lambda to the bacterial chromosome was demonstrated.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence DNA Transposable Elements DNA, Bacterial/analysis DNA, Viral/analysis Drug Resistance, Microbial Electrophoresis, Agar Gel Escherichia coli/genetics Genes Genetic Markers Kanamycin/pharmacology Recombination, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial DNA, Viral Genetic Markers Kanamycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Saint-Girons I
Fritz H J
Shaw C
Tillmann E
Starlinger P
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31 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270136
Published
1981-00-00
Pages
45-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270136
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