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PMID: 6278265 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the 3'-terminal region of rat 18S ribosomal DNA.

Molecular & general genetics : MGG ·Vol. 184 ·No. 3 ·1981-00-00 ·Pages 557-9

Torczynski R, Bollon AP, Fuke M

Abstract

The 3'-terminal 230 base-pairs (bp) of the gene for 18S rRNA and 40 bp of the adjoining spacer have been sequenced for the Sprague-Dawley rat. This mammalian sequence has been compared with the known sequences of yeast, fruit fly, silkworm, and frog. This study has shown that the nucleotide-sequence differences between rat and frog are the smallest among and longer species, probably reflecting their evolutionary closeness and longer maturation time compared to the others. There is little similarity in the nucleotide sequences of the transcribed spacer regions of the five species compared.

MeSH Terms

Animals Base Sequence DNA Restriction Enzymes Genes Molecular Weight RNA, Ribosomal/genetics Rats Species Specificity

Chemicals

RNA, Ribosomal DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Torczynski R

Bollon A P

Fuke M

References (12)

12 references, click to expand

The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.
Nucleic Acids Res. 1980 Oct 10;8(19):4365-76 PMID: 7433112
HindIII-sensitive sites present once in every four repeats of EcoRI-sensitive sites in Novikoff rat hepatoma DNA.
FEBS Lett. 1979 Mar 1;99(1):136-40 PMID: 437119
Sequence determination of 5'-terminal and 3'-terminal T1 oligonucleotides of 18-S ribosomal RNA of a mouse cell line (L 5178 Y).
Eur J Biochem. 1975 Jun 16;55(1):247-55 PMID: 170097
Introduction of specific cleavages into RNAs of RNA bacteriophages for determination of base sequences.
Proc Natl Acad Sci U S A. 1974 Mar;71(3):742-5 PMID: 4362631
Homology of the 3' terminal sequences of the 18S rRNA of Bombyx mori and the 16S rRNA of Escherchia coli.
Nucleic Acids Res. 1979 Nov 10;7(5):1109-19 PMID: 390496
Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.
Nucleic Acids Res. 1980 Dec 20;8(24):5993-6005 PMID: 6258158
Fine structure of ribosomal RNA. I. Conservation of homologous regions within ribosomal RNA of eukaryotes.
J Mol Biol. 1976 Sep 25;106(3):791-816 PMID: 978737
Sequence of the 3'-terminal portion of Drosophila melanogaster 18 S rRNA and of the adjoining spacer: comparison with corresponding prokaryotic and eukaryotic sequences.
FEBS Lett. 1980 Aug 11;117(1):227-31 PMID: 6250894
Characterization of cloned rat ribosomal DNA fragments.
Mol Gen Genet. 1981;182(1):25-30 PMID: 6455591
Evolutionary trends in 18S ribosomal RNA nucleotide sequences of rat, mouse, hamster and man.
Nucleic Acids Res. 1976 Nov;3(11):2939-57 PMID: 188015
Isolation from rat liver and sequence of a RNA fragment containing 32 nucleotides from position 5 to 36 from the 3' end of ribosomal 18S RNA.
Nucleic Acids Res. 1978 Feb;5(2):425-34 PMID: 634793
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00352540

Published

1981-00-00

Pages

557-9

Language

English

Region

Germany

NLM ID

0125036

Subset

Grants

NIGMS NIH HHS · GM-28090 · United States

Databases

GENBANK

K01589

External Links

PubMed source DOI: 10.1007/BF00352540

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Nucleotide sequence of the 3'-terminal region of rat 18S ribosomal DNA.

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