Home LiteratureArticle Details
PMID: 6289308 Published · ppublish English Journal Article

In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 15 ·1982-08-00 ·Pages 4609-12

Peacock S, Cenatiempo Y, Robakis N, Brot N, Weissbach H

Abstract

Plasmids pNF1337 and pNF1341, which contain part of the L10 operon including the RNA polymerase beta-subunit gene, have been used as templates in vitro to investigate expression of the beta-subunit gene. For these studies, the synthesis of the first dipeptide of the beta subunit, fMet-Val, was measured instead of that of the entire protein. By using this dipeptide system, we studied the effects of RNA polymerase holoenzyme and L factor (nus A gene product) on fMET-Val synthesis and compared the relative effects of the primary and secondary promoters in the L10 operon on expression of the beta-subunit gene. The results show that the inhibitory effect of RNA polymerase on beta-subunit synthesis and the stimulatory effect of L factor occur before formation of the first dipeptide bond. In this in vitro system, the secondary promoters account for about 50% of the total fMet-Val synthesized. Although the primary promoter is sensitive to guanosine 5'-diphosphate 3'-diphosphate in vitro, the secondary promoters are not affected by this nucleotide.

MeSH Terms
Bacterial Proteins/pharmacology Cell-Free System DNA, Bacterial/metabolism DNA-Directed RNA Polymerases/biosynthesis,pharmacology Dipeptides/biosynthesis Escherichia coli/enzymology Gene Expression Regulation/drug effects Macromolecular Substances Operon Plasmids
Chemicals
Bacterial Proteins DNA, Bacterial Dipeptides Macromolecular Substances DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Peacock S
Cenatiempo Y
Robakis N
Brot N
Weissbach H
References (30)
30 references, click to expand
  1. E. coli ribosomal protein L10 inhibits translation of L10 and L7/L12 mRNAs by acting at a single site.
    Nature. 1981 Nov 12;294(5837):190-2 PMID: 6272122
  2. In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.
    Mol Gen Genet. 1981;183(2):277-82 PMID: 7035827
  3. Negative regulation of beta and beta' synthesis by RNA polymerase.
    Mol Gen Genet. 1981;183(3):514-7 PMID: 6460912
  4. Use of different tRNASer isoacceptor species in vitro to discriminate between the expression of plasmid genes.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1466-8 PMID: 6803242
  5. Methionyl soluble ribonucleic acid transformylase. I. Purification and partial characterization.
    J Biol Chem. 1967 Apr 10;242(7):1522-5 PMID: 5337045
  6. Structure and orientation of an RNA polymerase operon in Escherichia coli.
    Nature. 1974 Jun 7;249(457):519-22 PMID: 4599760
  7. Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase.
    Mol Gen Genet. 1974;132(3):193-201 PMID: 4607989
  8. Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.
    J Biol Chem. 1975 Feb 25;250(4):1556-62 PMID: 1089661
  9. DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3156-9 PMID: 787986
  10. Biosynthetic regulation of individual proteins in relA+ and relA strains of Escherichia coli during amino acid starvation.
    Mol Gen Genet. 1976 Dec 22;149(3):279-89 PMID: 799246
  11. The effects of the relA gene on the synthesis of aminoacyl-tRNA synthetases and other transcription and translation proteins in Escherichia coli A.
    Mol Gen Genet. 1976 Dec 22;149(3):291-6 PMID: 799247
  12. In vivo transcription of E. coli genes coding for rRNA, ribosomal proteins and subunits of RNA polymerase: influence of the stringent control system.
    Mol Gen Genet. 1977 Oct 20;155(2):203-11 PMID: 337129
  13. Autogenous regulation of RNA polymerase beta subunit synthesis in vitro.
    J Biol Chem. 1978 Jul 10;253(13):4501-4 PMID: 350875
  14. The relationship between the spoT gene, the synthesis of stable RNA, ribosomal proteins, and the beta beta' subunits of RNA polymerase following a nutritional shiftup of Escherichia coli.
    Can J Biochem. 1978 Jun;56(6):528-33 PMID: 352488
  15. Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3891-5 PMID: 358203
  16. Identification of a single promoter in E. coli for rplJ, rplL and rpoBC.
    Nature. 1978 Nov 2;276(5683):33-7 PMID: 368640
  17. Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697-701 PMID: 377281
  18. In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1716-20 PMID: 287012
  19. Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.
    Mol Gen Genet. 1979 May 23;173(1):39-50 PMID: 381841
  20. Control features within the rplJL-rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4922-6 PMID: 116224
  21. DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6115-9 PMID: 160561
  22. Regulation of the in vitro synthesis of E. coli ribosomal protein L12.
    Biochem Biophys Res Commun. 1979 Dec 28;91(4):1453-61 PMID: 160796
  23. L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1991-4 PMID: 6154941
  24. Bacteriophage lambda vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2124-8 PMID: 6445564
  25. Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2592-5 PMID: 6994102
  26. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
  27. Autogenous and post-transcriptional regulation of Escherichia coli RNA polymerase synthesis in vitro.
    Mol Gen Genet. 1980;179(3):489-96 PMID: 6160369
  28. Pausing and termination of transcription within the early region of bacteriophage T7 DNA in vitro.
    J Biol Chem. 1981 Mar 25;256(6):2777-86 PMID: 7009597
  29. The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
    Eur J Biochem. 1981 Jun 1;116(3):621-9 PMID: 6266829
  30. Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4261-4 PMID: 7027258
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.15.4609
Published
1982-08-00
Pages
4609-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346724
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.15.4609 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2