In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.
Sucrose density gradient centrifugation and DNA/RNA hybridization have been used to analyse the mRNA synthesized from the ribosomal protein - RNA polymerase subunits gene cluster rplKAJL-rpoBC in Escherichia coli. DNA/RNA hybrids obtained from total E. coli RNA and specific DNA restriction fragments from this chromosomal area were further subjected to endonuclease S1 digestion. This analysis permits the mapping of the ends of mRNA molecules for specific genes or operons by sizing the S1 resistant hybrids. Our results show that the predominant mRNA synthesized under conditions of balanced growth from the rplKAJL-rpoBC region codes for the four ribosomal proteins L11, L1, L10 and L7/12. This tetracistronic mRNA puts the transcription of the following rpoBC genes under the main control of the L11 promoter. Smaller distinct mRNA species could also be detected by this technique. They originate from intercistronic transcription termination and re-initiation as well as from processing of the larger polycistronic mRNA.
28 references, click to expand
-
Transcription patterns of adjacent segments on the chromosome of Escherichia coli containing genes coding for four 50S ribosomal proteins and the beta and beta' subunits of RNA polymerase.J Mol Biol. 1977 Oct 5;115(4):603-25 PMID: 338915
-
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
-
Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.Mol Gen Genet. 1980 Feb;177(3):527-33 PMID: 6246404
-
Regulation of biosynthesis of the DNA-dependent RNA polymerase in Escherichia coli.Curr Top Cell Regul. 1980;17:89-136 PMID: 7002486
-
Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
-
Linkage map of Escherichia coli K-12, edition 6.Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
-
Evidence for co-transcription of the RNA polymerase genes rpoBC with a ribosomal protein gene of escherichia coli.Mol Gen Genet. 1979 Jan 31;169(2):195-204 PMID: 375008
-
Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome.J Bacteriol. 1979 Jan;137(1):584-94 PMID: 368032
-
Genetics of bacterial ribosomes.Annu Rev Genet. 1977;11:297-347 PMID: 339818
-
Spliced early mRNAs of simian virus 40.Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
-
Feedback regulation of ribosomal protein gene expression in Escherichia coli.Proc Natl Acad Sci U S A. 1980 Jun;77(6):3590-4 PMID: 6251471
-
Deletions, insertions and rearrangements affecting rpoB gene expression.Mol Gen Genet. 1979 Jun 7;173(2):217-20 PMID: 386038
-
Transcriptional and post-transcriptional control of RNA polymerase and ribosomal protein genes cloned on composite ColE1 plasmids in the bacterium Escherichia coli.J Biol Chem. 1979 Aug 25;254(16):7540-7 PMID: 381287
-
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697-701 PMID: 377281
-
Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.Mol Gen Genet. 1979 May 23;173(1):39-50 PMID: 381841
-
Organization of bacterial genes for ribosomal components: studies using novel approaches.Cell. 1976 Dec;9(4 PT 2):633-44 PMID: 797458
-
Autogenous regulation of the synthesis of ribosomal proteins, L10 and L7/12, in Escherichia coli.Mol Gen Genet. 1980;178(2):483-6 PMID: 6993849
-
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.Eur J Biochem. 1973 Feb 15;33(1):192-200 PMID: 4691350
-
Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.Cell. 1977 Feb;10(2):265-73 PMID: 837449
-
Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli.Proc Natl Acad Sci U S A. 1978 Aug;75(8):3891-5 PMID: 358203
-
In vitro expression of Escherichia coli ribosomal protein genes: autogenous inhibition of translation.Proc Natl Acad Sci U S A. 1980 Apr;77(4):1837-41 PMID: 6445562
-
[Primary structure of Escherichia coli RNA-polymerase. Nucleotide sequence of gene rpoB and amino acid sequence of the beta-subunit].Dokl Akad Nauk SSSR. 1980;253(4):994-8 PMID: 7000477
-
Mapping of a cluster of genes for components of the transcriptional and translational machineries of Escherichia coli.J Mol Biol. 1977 Jan 5;109(1):23-47 PMID: 320344
-
Feedback regulation of ribosomal protein gene expression in Escherichia coli: structural homology of ribosomal RNA and ribosomal protein MRNA.Proc Natl Acad Sci U S A. 1980 Dec;77(12):7084-8 PMID: 7012833
-
Identification of a single promoter in E. coli for rplJ, rplL and rpoBC.Nature. 1978 Nov 2;276(5683):33-7 PMID: 368640
-
Control features within the rplJL-rpoBC transcription unit of Escherichia coli.Proc Natl Acad Sci U S A. 1979 Oct;76(10):4922-6 PMID: 116224
-
Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.Proc Natl Acad Sci U S A. 1980 May;77(5):2592-5 PMID: 6994102
-
Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.J Bacteriol. 1973 Nov;116(2):517-26 PMID: 4583237
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