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PMID: 6292456 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detailed characterization of an apparently unspliced beta herpes simplex virus type 1 gene mapping in the interior of another.

Journal of virology ·Vol. 43 ·No. 3 ·1982-09-00 ·Pages 1123-8

Draper KG, Frink RJ, Wagner EK

Abstract

We precisely localized the coding region and determined the nucleotide sequence of a 1.2-kilobase beta herpes simplex virus type 1 mRNA which underlies the 3' region of the 5.2-kilobase beta mRNA mapping in HindIII fragment K. This mRNA, which lacks readily detectable splices, has its own promoter by the criteria of identification of putative herpes simplex virus type 1 control sequences and in vitro transcription by a Manley polymerase system.

MeSH Terms
Base Sequence DNA Restriction Enzymes Genes, Viral RNA Splicing RNA, Messenger/genetics RNA, Viral/genetics Simplexvirus/genetics Transcription, Genetic
Chemicals
RNA, Messenger RNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Draper K G
Frink R J
Wagner E K
References (23)
23 references, click to expand
  1. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
    Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
  2. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  3. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
    Cell. 1977 May;11(1):223-32 PMID: 194704
  4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  5. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  6. X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I.
    Anal Biochem. 1978 May;86(1):184-92 PMID: 655381
  7. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  8. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  10. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  11. Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10 PMID: 6255460
  12. Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.
    Nucleic Acids Res. 1980 Dec 20;8(24):5931-48 PMID: 6258155
  13. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
  14. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  15. The SV40 early region TATA box is required for accurate in vitro initiation of transcription.
    Nature. 1981 Mar 26;290(5804):310-5 PMID: 6259539
  16. Detailed characterization of the mRNA mapping in the HindIII fragment K region of the herpes simplex virus type 1 genome.
    J Virol. 1981 Mar;37(3):1011-27 PMID: 6262521
  17. Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441-5 PMID: 6262799
  18. Characterization of a major late herpes simplex virus type 1 mRNA.
    J Virol. 1981 May;38(2):483-96 PMID: 6264123
  19. Herpes simplex virus type 1 HindIII fragment L encodes spliced and complementary mRNA species.
    J Virol. 1981 Aug;39(2):559-72 PMID: 6268843
  20. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  21. Uninfected cell polymerase efficiently transcribes early but not late herpes simplex virus type 1 mRNA.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6139-43 PMID: 6273852
  22. Identification of proteins encoded by a fragment of herpes simplex virus type 2 DNA that has transforming activity.
    J Virol. 1982 May;42(2):530-7 PMID: 6283171
  23. Herpes simplex virus mRNA species mapping in EcoRI fragment I.
    J Virol. 1982 Aug;43(2):594-607 PMID: 6287033
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.3.1123-1128.1982
Published
1982-09-00
Pages
1123-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256225
Subset
IM
Grants
NCI NIH HHS · CA11861 · United States
Databases
GENBANK
J02212
External Links
PubMed source DOI: 10.1128/JVI.43.3.1123-1128.1982 PMC Full Text View journal details
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