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PMID: 6292522 Published · ppublish English Journal Article

Cloning of endogenous murine leukemia virus-related sequences from chromosomal DNA of BALB/c and AKR/J mice: identification of an env progenitor of AKR-247 mink cell focus-forming proviral DNA.

Journal of virology ·Vol. 44 ·No. 2 ·1982-11-00 ·Pages 625-36

Khan AS, Rowe WP, Martin MA

Abstract

Recombinant phages containing murine leukemia virus (MuLV)-reactive DNA sequences were isolated after screening of a BALB/c mouse embryo DNA library and from shotgun cloning of EcoRI-restricted AKR/J mouse liver DNA. Twelve different clones were isolated which contained incomplete MuLV proviral DNA sequences extending various distances from either the 5' or 3' long terminal repeat (LTR) into the viral genome. Restriction maps indicated that the endogenous MuLV DNAs were related to xenotropic MuLVs, but they shared several unique restriction sites among themselves which were not present in known MuLV proviral DNAs. Analyses of internal restriction fragments of the endogenous LTRs suggested the existence of at least two size classes, both of which were larger than the LTRs of known ecotropic, xenotropic, or mink cell focus-forming (MCF) MuLV proviruses. Five of the six cloned endogenous MuLV proviral DNAs which contained envelope (env) DNA sequences annealed to a xenotropic MuLV env-specific DNA probe; in addition, four of these five also hybridized to an ecotropic MuLV-specific env DNA probe. Cloned MCF 247 proviral DNA also contained such dual-reactive env sequences. One of the dual-reactive cloned endogenous MuLV DNAs contained an env region that was indistinguishable by AluI and HpaII digestion from the analogous segment in MCF 247 proviral DNA and may therefore represent a progenitor for the env gene of this recombinant MuLV. In addition, the endogenous MuLV DNAs were highly related by AluI cleavage to the Moloney MuLV provirus in the gag and pol regions.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes, Viral Leukemia Virus, Murine/genetics Mice Mice, Inbred AKR/genetics Mice, Inbred BALB C/genetics Mice, Inbred Strains/genetics Recombination, Genetic Repetitive Sequences, Nucleic Acid Viral Envelope Proteins Viral Proteins/genetics
Chemicals
Viral Envelope Proteins Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Khan A S
Rowe W P
Martin M A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.2.625-636.1982
Published
1982-11-00
Pages
625-36
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256306
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.44.2.625-636.1982 PMC Full Text View journal details
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