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PMID: 6294057 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Escherichia coli mutants defective in the uncH gene.

Journal of bacteriology ·Vol. 153 ·No. 1 ·1983-01-00 ·Pages 416-22

Humbert R, Brusilow WS, Gunsalus RP, Klionsky DJ, Simoni RD

Abstract

Plasmids carrying cloned segments of the unc operon of Escherichia coli have been used in genetic complementation analyses to identify three independent mutants defective in the uncH gene, which codes for the delta subunit of the ATP synthetase. Mutations in other unc genes have also been mapped by this technique. ATPase activity was present in extracts of the uncH mutants, but the enzyme was not as tightly bound to the membrane as it was in the parental strain. ATP-dependent membrane energization was absent in membranes isolated from the uncH mutants and could not be restored by adding normal F1 ATPase from the wild-type strain. F1 ATPase prepared from uncH mutants could not restore ATP-dependent membrane energization when added to wild-type membranes depleted of F1. Membranes of the uncH mutants were not rendered proton permeable as a result of washing with low-ionic-strength buffer.

MeSH Terms
ATP Synthetase Complexes Adenosine Triphosphatases/metabolism Adenosine Triphosphate/metabolism Cell Membrane/enzymology Escherichia coli/enzymology,genetics Genes Genetic Complementation Test Multienzyme Complexes/genetics Mutation NAD/metabolism Phosphotransferases/genetics Plasmids
Chemicals
Multienzyme Complexes NAD Adenosine Triphosphate Phosphotransferases ATP Synthetase Complexes Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Humbert R
Brusilow W S
Gunsalus R P
Klionsky D J
Simoni R D
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.1.416-422.1983
Published
1983-01-00
Pages
416-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217388
Subset
IM
Grants
NIGMS NIH HHS · GM07276 · United States
NIGMS NIH HHS · GM07598 · United States
NIGMS NIH HHS · GM18539 · United States
External Links
PubMed source DOI: 10.1128/jb.153.1.416-422.1983 PMC Full Text View journal details
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