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PMID: 6296052 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction of hybrid plasmids containing the Escherichia coli uxaB gene: analysis of its regulation and direction of transcription.

Journal of bacteriology ·Vol. 153 ·No. 2 ·1983-02-00 ·Pages 747-55

Blanco C, Mata-Gilsinger M, Ritzenthaler P

Abstract

The uxaB gene of Escherichia coli, encoding for altronate oxidoreductase involved in the hexuronate degradative pathway, was isolated on a ColE1-uxaB hybrid plasmid from the Clarke and Carbon bank. The restriction map of this plasmid was established. The uxaB gene was mapped on a 1.5-megadalton HindIII-KpnI DNA fragment. Use of an in vitro gene fusion between uxaB and lacZ genes led to the determination that uxaB is transcribed from the KpnI towards the HindIII restriction sites. Gene amplification in cells containing various uxaB hybrid plasmids allowed us to show a gradation in the level of repression of exu operator sites by the exuR regulatory gene product.

MeSH Terms
Carbohydrate Dehydrogenases/biosynthesis,genetics Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Escherichia coli/enzymology,genetics Genes, Bacterial Genes, Regulator Operon Plasmids Transcription, Genetic
Chemicals
DNA, Recombinant Carbohydrate Dehydrogenases tagaturonate reductase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blanco C
Mata-Gilsinger M
Ritzenthaler P
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.2.747-755.1983
Published
1983-02-00
Pages
747-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221693
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.153.2.747-755.1983 PMC Full Text View journal details
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