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PMID: 6298722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria.

Nucleic acids research ·Vol. 11 ·No. 2 ·1983-01-25 ·Pages 339-48

Sor F, Fukuhara H

Abstract

The mitochondrial gene coding for the large ribosomal RNA (21S) has been isolated from a rho- clone of Saccharomyces cerevisiae. A DNA segment of about 5500 base pairs has been sequenced which included the totality of the sequence coding for the mature ribosomal RNA and the intron. The mature RNA sequence corresponds to a length of 3273 nucleotides. Despite the very low guanine-cytosine content (20.5%), many stretches of sequence are homologous to the corresponding Escherichia coli 23S ribosomal RNA. The sequence can be folded into a secondary structure according to the general models for prokaryotic and eukaryotic large ribosomal RNAs. Like the E.coli gene, the mitochondrial gene contains the sequences that look like the eukaryotic 5.8S and the chloroplastic 4.5S ribosomal RNAs. The 5' and 3' end regions show a complementarity over fourteen nucleotides.

MeSH Terms
Base Sequence DNA/genetics DNA Restriction Enzymes DNA Transposable Elements DNA, Mitochondrial/genetics DNA, Ribosomal Genes Mitochondria/analysis Mutation Nucleic Acid Conformation RNA, Ribosomal/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA Transposable Elements DNA, Mitochondrial DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sor F
Fukuhara H
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20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.2.339
Published
1983-01-25
Pages
339-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325718
Subset
IM
Databases
GENBANK
J01526, J01527, J01528, V00684, V00688, V00689, V00699
External Links
PubMed source DOI: 10.1093/nar/11.2.339 PMC Full Text View journal details
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