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PMID: 6299943 Published · ppublish English Journal Article

Enhanced superoxide anion release from phagocytes by muramyl dipeptide or lipopolysaccharide.

Infection and immunity ·Vol. 39 ·No. 2 ·1983-02-00 ·Pages 559-64

Kaku M, Yagawa K, Nagao S, Tanaka A

Abstract

Muramyl dipeptide (MDP) and lipopolysaccharide (LPS) from Escherichia coli were tested for the ability to influence superoxide anion (O2-) release from guinea pig phagocytes. Both MDP and LPS alone did not, by themselves, stimulate O2- release by macrophages and polymorphonuclear leukocytes. However, the preincubation of macrophages with MDP or LPS primed the macrophages to release an enhanced amount of O2- when stimulated by cytochalasin E and wheat germ agglutinin. When polymorphonuclear leukocytes were treated in the same way, only LPS showed an enhancing effect. MDP enhanced NADPH oxidase activity of macrophages, which is probably the reason for enhanced O2- release by MDP.

MeSH Terms
Acetylmuramyl-Alanyl-Isoglutamine/pharmacology Animals Dose-Response Relationship, Drug Escherichia coli Guinea Pigs Kinetics Lectins Lipopolysaccharides/pharmacology Lymphocytes/physiology Macrophages/drug effects,metabolism Male NADH, NADPH Oxidoreductases/metabolism NADPH Oxidases Neutrophils/drug effects,metabolism Oxygen/metabolism Superoxides/metabolism Wheat Germ Agglutinins
Chemicals
Lectins Lipopolysaccharides Wheat Germ Agglutinins Superoxides Acetylmuramyl-Alanyl-Isoglutamine NADH, NADPH Oxidoreductases NADPH Oxidases Oxygen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaku M
Yagawa K
Nagao S
Tanaka A
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29 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.39.2.559-564.1983
Published
1983-02-00
Pages
559-64
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347987
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.39.2.559-564.1983 PMC Full Text View journal details
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