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PMID: 6300788 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure of the dihydrofolate reductase gene in Chinese hamster ovary cells.

Nucleic acids research ·Vol. 11 ·No. 7 ·1983-04-11 ·Pages 1997-2012

Carothers AM, Urlaub G, Ellis N, Chasin LA

Abstract

Overlapping recombinant lambda 1059 phages carrying regions of the dhfr locus from the amplified Chinese hamster ovary (CHO) cell clone MK42 have been isolated. In addition, dhfr cDNAs from this cell line have been cloned into plasmid pBR322. Restriction analysis of these recombinant molecules has led to a map of the Chinese hamster dhfr gene. This gene has a minimum size of 26 kb and contains six exons as defined by hybridization to a combination of mouse and CHO cDNA probes. The latter probes reveal 3' exonic sequences that are not present in mouse cDNA. The CHO dhfr gene thus extends about 700 bp further 3' than in the mouse, consistent with the larger size of the hamster mRNA. At least five intervening sequences are present, of approximate sizes: 0.3, 2.5, 8.6, 2.6 and 9.4 kb. Four sequences from highly repeated families are situated in introns within the dhfr gene. The overall structure of this gene is strikingly similar to that of the mouse. Evolutionary conservation of interrupted gene structure among mammals thus extends to genes that code for household enzymes as well as specialized or structural proteins.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Cell Line Cricetinae Cricetulus DNA/isolation & purification DNA Restriction Enzymes DNA, Recombinant/metabolism Female Genes Nucleic Acid Hybridization Ovary Tetrahydrofolate Dehydrogenase/genetics
Chemicals
DNA, Recombinant DNA Tetrahydrofolate Dehydrogenase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carothers A M
Urlaub G
Ellis N
Chasin L A
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.7.1997
Published
1983-04-11
Pages
1997-2012
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325857
Subset
IM
Grants
NIGMS NIH HHS · GM34537 · United States
External Links
PubMed source DOI: 10.1093/nar/11.7.1997 PMC Full Text View journal details
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