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PMID: 6287224 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Direct demonstration of genetic alterations at the dihydrofolate reductase locus after gamma irradiation.

Molecular and cellular biology ·Vol. 2 ·No. 1 ·1982-01-00 ·Pages 93-6

Graf LH, Chasin LA

Abstract

Gamma ray-induced mutants of Chinese hamster ovary cells lacking dihydrofolate reductase activity were screened for DNA sequence changes at the locus specifying this activity by using a cloned cDNA probe. Two of nine mutants screened displayed an altered restriction fragment pattern suggesting the occurrence of DNA deletions or rearrangements.

MeSH Terms
Animals Base Sequence Cells, Cultured Chromosome Aberrations Chromosome Deletion Cricetinae Cricetulus DNA Restriction Enzymes Gamma Rays Nucleic Acid Hybridization Tetrahydrofolate Dehydrogenase/genetics
Chemicals
Tetrahydrofolate Dehydrogenase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Graf L H
Chasin L A
References (24)
24 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.1.93-96.1982
Published
1982-01-00
Pages
93-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369757
Subset
IM
Grants
NIGMS NIH HHS · GM22629 · United States
External Links
PubMed source DOI: 10.1128/mcb.2.1.93-96.1982 PMC Full Text View journal details
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