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PMID: 6304468 Published · ppublish English Journal Article

Tn2610, a transposon involved in the spread of the carbenicillin-hydrolyzing beta-lactamase gene.

Molecular & general genetics : MGG ·Vol. 189 ·No. 2 ·1983-00-00 ·Pages 282-8

Yamamoto T, Watanabe M, Matsumoto K, Sawai T

Abstract

We have found a new transposon, Tn2610, on pCS200 in clinical isolates of Escherichia coli, which encodes the carbenicillin-hydrolyzing beta-lactamase gene in combination with the resistance determinants to streptomycin and sulfonamide. Tn2610 has a molecular size of 24 kilobase pairs and is flanked by long inverted repeat sequences of 3 kilobase pairs in length. Genetical and physical analyses indicate that Tn2610 is a single transposable unit encoding the multiple resistance determinants and that is different from any previously described transposon. The characteristic DNA structure observed in various complex resistance transposons involved in the transposition of the carbenicillin-hydrolyzing beta-lactamase gene is discussed.

MeSH Terms
Carbenicillin Chromosome Mapping DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics Genes Genes, Bacterial Penicillin Resistance Plasmids Repetitive Sequences, Nucleic Acid beta-Lactamases/genetics
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes beta-Lactamases Carbenicillin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamamoto T
Watanabe M
Matsumoto K
Sawai T
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26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337818
Published
1983-00-00
Pages
282-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337818
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