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PMID: 6308626 Published · ppublish English Journal Article

Molecular cloning and characterization of the gene coding for human complement protein factor B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 14 ·1983-07-00 ·Pages 4464-8

Campbell RD, Porter RR

Abstract

Four cosmid clones, each with an average insert size of 40 kilobase pairs and containing the factor B gene, were isolated from a human genomic DNA library. The clones were identified by hybridization with a 515-base-pair cDNA probe isolated by using a unique 17-base synthetic oligonucleotide probe from a human liver cDNA library. The cosmid clones were characterized by restriction endonuclease digestion and Southern blotting, and a partial restriction map of the DNA represented in the cosmids was constructed. The Bb portion of the factor B gene is about 4 kb in length. DNA sequence analysis has resulted in the determination of 3.3 kb of sequence at the 3' end of the gene. This region codes for amino acids 87-505 of Bb and includes the whole of the serine proteinase domain of the protein. The three active site residues of histidine, aspartic acid, and serine found at positions 267, 317, and 440 of the Bb sequence, respectively, lie on separate exons. Other functional regions within the serine proteinase domain are separated also by intervening sequences.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes, Human, 6-12 and X Cloning, Molecular Complement Factor B/genetics DNA/analysis DNA Restriction Enzymes Enzyme Precursors/genetics Genes Humans Major Histocompatibility Complex
Chemicals
Enzyme Precursors DNA DNA Restriction Enzymes Complement Factor B
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Campbell R D
Porter R R
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.14.4464
Published
1983-07-00
Pages
4464-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384059
Subset
IM
Databases
GENBANK
J00125, J00126
External Links
PubMed source DOI: 10.1073/pnas.80.14.4464 PMC Full Text View journal details
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