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PMID: 6310493 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A comparison of the promoter strengths of two eukaryotic genes in vitro reveals a region of DNA that can influence the rate of transcription in cis over long distances.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5317-26

Gregory SP, Butterworth PH

Abstract

We have compared the strength of a trout protamine gene promoter with that of the mouse beta major-globin gene by analysing the relative levels of run-off transcripts produced in a single mammalian in vitro transcription reaction. When the promoters are introduced on separate recombinant plasmids, the protamine transcripts are synthesised with much greater efficiency than those originating from the globin cap site. This enhanced transcription of the protamine gene is again observed when the promoters are applied as separate DNA fragments derived from the same recombinant plasmid. However, when the promoters are linked on a DNA fragment that includes 7 kb of DNA separating the initiation sites, then there is a marked reduction in the protamine signal relative to the globin. Deletion of a region of this fragment that contains the sequences flanking the globin gene at positions -335 to -1400 restores the enhanced protamine gene expression to the levels observed when the promoters are carried on separate DNA fragments.

MeSH Terms

Animals Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Globins/genetics Mice Nucleic Acid Hybridization Operon Protamines/genetics Species Specificity Transcription, Genetic Trout

Chemicals

DNA, Recombinant Protamines Globins DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gregory S P

Butterworth P H

References (22)

22 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.16.5317

Published

1983-08-25

Pages

5317-26

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326280

Subset

External Links

PubMed source DOI: 10.1093/nar/11.16.5317 PMC Full Text View journal details

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A comparison of the promoter strengths of two eukaryotic genes in vitro reveals a region of DNA that can influence the rate of transcription in cis over long distances.

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