Nucleotide sequence of the constant region of a chicken mu heavy chain immunoglobulin mRNA.
We have recently reported the sequence of a chicken Ig lambda light chain cDNA clone, isolated from a spleen partial cDNA library (1). In this paper, we describe the characterization of a cDNA clone coding for the chicken constant (C) region of the secreted mu chain. This is the first report on the nucleotide and amino acid sequence of a chicken Ig heavy chain constant region. It contains the 3' untranslated region of the mu mRNA up to the poly(A) tail, and, in comparison with the mouse Cmu sequence, displays the overall domain size and organization of a secreted mu chain, i.e.: a characteristic COOH-terminal region, a Cmu4, a Cmu3, a Cmu2, and part of a Cmu1 domain. The sequence homology between these two species ranges from 45% for the Cmu4 to 18% for the Cmu2. Thus, the Cmu sequence appears much less conserved between chicken and mouse than their respective lambda light chain constant regions (1). These results, together with some distinctive features of the Cmu2 domain, may be of evolutionary relevance and will be further discussed.
25 references, click to expand
-
Immunoglobulin lambda-chains. The complete amino acid sequence of a Bence-Jones protein.Biochem J. 1968 Dec;110(4):631-52 PMID: 4883841
-
Studies on the selectivity of DNA precipitation by spermine.Nucleic Acids Res. 1981 Oct 24;9(20):5493-504 PMID: 7029471
-
Complete amino acid sequence of the Mu heavy chain of a human IgM immunoglobulin.Science. 1973 Oct 19;182(4109):287-91 PMID: 4742735
-
The general structure of shark (Squalis acantias) and hen (Galus domesticus) immunoglobulins.Mol Biol Rep. 1974 Mar;1(6):301-4 PMID: 4427593
-
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
-
Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark.Biochemistry. 1976 Jul 27;15(15):3379-90 PMID: 986160
-
Ontogeny of B cells in the chicken. II. Changing patterns of cytoplasmic IgM expression and of modulation requirements for surface IgM by anti-mu antibodies.J Immunol. 1977 Aug;119(2):749-56 PMID: 407304
-
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
-
Amino acid sequence of the Fc region of a canine immunoglobulin M: interspecies homology for the IgM class.Science. 1978 Jun 9;200(4346):1159-61 PMID: 653360
-
pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
-
Rapid purification of plasmid DNAs by hydroxyapatite chromatography.Eur J Biochem. 1978 Nov 2;91(1):303-10 PMID: 363426
-
Sequences of mouse immunoglobulin light chain genes before and after somatic changes.Cell. 1978 Dec;15(4):1133-44 PMID: 103630
-
Amino acid sequence of a mouse immunoglobulin mu chain.Proc Natl Acad Sci U S A. 1979 Jun;76(6):2932-6 PMID: 111247
-
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
-
In vitro replication of infectious bursal disease virus in established lymphoid cell lines and chicken B lymphocytes.Infect Immun. 1979 Sep;25(3):964-70 PMID: 227789
-
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
-
Organization and reorganization of immunoglobulin genes in A-MULV-transformed cells: rearrangement of heavy but not light chain genes.Cell. 1981 Dec;27(2 Pt 1):381-90 PMID: 6277505
-
Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
-
Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.Cell. 1980 Jun;20(2):293-301 PMID: 6771018
-
Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.Cell. 1980 Jun;20(2):303-12 PMID: 6771019
-
Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.Cell. 1980 Aug;21(1):179-87 PMID: 6157477
-
Immunoglobulin gene rearrangement in immature B cells.Science. 1980 Sep 19;209(4463):1366-9 PMID: 6774416
-
Sequence of the gene for the constant region of the mu chain of Balb/c mouse immunoglobulin.Gene. 1981 Oct;15(1):33-42 PMID: 6795090
-
Human immunoglobulin heavy chain genes: evolutionary comparisons of C mu, C delta and C gamma genes and associated switch sequences.Nucleic Acids Res. 1981 Sep 25;9(18):4509-24 PMID: 6795593
-
Conformation of the free and antigen-bound IgM antibody molecules.Nature. 1969 Dec 27;224(5226):1307-9 PMID: 5359295
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
