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PMID: 6310498 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 5'-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5403-12

Ohno S, Taniguchi T

Abstract

The structural gene for Herpes simplex virus (HSV) thymidine kinase (Tk) was fused downstream of the 5'-flanking sequence (from -284 to +20; numbering relative to the putative transcription initiation site) of the cloned human interferon-beta 1 (IFN-beta 1) gene. The fusion gene was linked to the vector pSV2-Ecogpt and the recombinant plasmid was used to transform mouse FM3A cells. All cloned transformants in which the fusion gene was integrated in an intact form produced the Tk specific transcript with the distinct 5' terminus corresponding to that of the authentic IFN-beta 1 mRNA when they were exposed to Newcastle disease virus (NDV). Thus, the results reported here provide evidence for the presence of specific DNA sequences in the 5'-flanking region of the IFN-beta 1 gene required for the virus mediated activation of transcription.

MeSH Terms
Animals Cell Line Cell Transformation, Viral DNA, Recombinant Endonucleases Genes Genes, Viral Genetic Vectors Humans Interferon-gamma/genetics Mice Nucleic Acid Hybridization Plasmids Simplexvirus/genetics Single-Strand Specific DNA and RNA Endonucleases Thymidine Kinase/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Interferon-gamma Thymidine Kinase Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohno S
Taniguchi T
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5403
Published
1983-08-25
Pages
5403-12
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326286
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.16.5403 PMC Full Text View journal details
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