Maintenance and incompatibility of plasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5775-91

Stuitje AR, Meijer M

Abstract

Plasmids that replicate only by means of the cloned Escherichia coli replication origin (oriC) are called minichromosomes or oriC-plasmids. In this paper it is shown that sequences located between oriC and asnA are involved in maintenance and incompatibility of minichromosomes. These sequences include part of the 16kD and 17kD genes, previously allocated within this region (1,2). Transcription towards oriC that is initiated at the 16kD promoter, specifically enhances the stability and copy-number of minichromosomes. Three regions are involved in minichromosome incompatibility. One region, incA, includes the minimal oriC sequence. A second, incB, maps within a 210 base pairs fragment that overlaps the 16kD promoter. The third, incC, encompasses the 17kD gene. Neither one of the regions expresses incompatibility on its own, but the additional presence of one of the others is required. The data presented indicate that sequences of the 16kD and 17kD genes are part of the replication control system of oriC-plasmids.

MeSH Terms

Base Sequence Chromosomes, Bacterial/metabolism Cloning, Molecular DNA Replication DNA Restriction Enzymes Escherichia coli/genetics,growth & development Genes, Bacterial Kinetics Operon Plasmids Transcription, Genetic

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stuitje A R

Meijer M

References (28)

28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.16.5775

Published

1983-08-25

Pages

5775-91

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326313

Subset

External Links

PubMed source DOI: 10.1093/nar/11.16.5775 PMC Full Text View journal details

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Maintenance and incompatibility of plasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA.

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