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PMID: 6312415 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells.

Nucleic acids research ·Vol. 11 ·No. 18 ·1983-09-24 ·Pages 6243-54

Carter AT, Norton JD, Avery RJ

Abstract

A family of dispersed, moderately repeated mouse genetic elements is expressed as retrovirus-like 30S RNA species (VL30 RNA) which can be transmitted to other cells when packaged as a pseudovirion complex by murine leukemia viruses (MuLV). Using the endogenous reverse transcriptase reaction of VL30 RNA-containing MuLV particles, full-length VL30 DNA was synthesized and cloned in pAT153. Analysis of a number of clones identified long terminal repeat structures (LTRs) characteristic of retrovirus proviruses and transposable genetic elements. Whilst the unique region of all clones was identical, the LTRs displayed some heterogeneity. Comparison of the unique region of cloned VL30 DNA with mouse genomic VL30 sequences showed the retrovirus-derived clones to be encoded by only a few members of the divergent VL30 gene family. These findings thus demonstrate a method for cloning a defined sub-class of retrovirus-like cellular genes which are both transcriptionally active and transmissible by a retrovirus.

MeSH Terms

Animals Cells, Cultured Cloning, Molecular DNA/isolation & purification DNA Replication DNA, Viral/genetics,isolation & purification Leukemia Virus, Murine/genetics Mice Nucleic Acid Hybridization RNA, Viral/genetics,isolation & purification Retroviridae/genetics Transcription, Genetic

Chemicals

DNA, Viral RNA, Viral DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Carter A T

Norton J D

Avery R J

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.18.6243

Published

1983-09-24

Pages

6243-54

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326370

Subset

External Links

PubMed source DOI: 10.1093/nar/11.18.6243 PMC Full Text View journal details

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A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells.

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