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PMID: 221684 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Defective retrovirus-like 30S RNA species of rat and mouse cells are infectious if packaged by type C helper virus.

Journal of virology ·Vol. 29 ·No. 3 ·1979-03-00 ·Pages 964-72

Scolnick EM, Vass WC, Howk RS, Duesberg PH

Abstract

RNA species with properties of defective retrovirus-like 30S RNA genomes have previously been detected in both rats and mice and in some rat and mouse retroviruses. Using cell lines which express high levels of this retrovirus-like RNA, we formed pseudotypes of the 30S RNAs with helper-independent type C viruses. A pseudotype virus complex containing a mouse 30S subunit was transmitted to rat cells, and a pseudotype virus complex containing a rat 30S subunit was transmitted to bat cells. In other transmission experiments, a rat 30S subunit was isolated in nonproducer bat cells without detectable expression of the helper-independent type C virus used to pseudotype it. The results provide further support for the retrovirus-like nature of the rat 30S subunit and provide evidence which supports the protovirus hypothesis proposed by Temin.

MeSH Terms
Animals Cell Line Chiroptera Helper Viruses/genetics,growth & development Hybridization, Genetic Mice Moloney murine leukemia virus/genetics,growth & development RNA, Viral/genetics Rats Retroviridae/genetics,growth & development Virus Replication
Chemicals
RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Scolnick E M
Vass W C
Howk R S
Duesberg P H
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.3.964-972.1979
Published
1979-03-00
Pages
964-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353256
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.3.964-972.1979 PMC Full Text View journal details
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