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PMID: 178897 Published · ppublish English Journal Article

Characterizatiion of rat genetic sequences of Kirsten sarcoma virus: distinct class of endogenous rat type C viral sequences.

Journal of virology ·Vol. 18 ·No. 2 ·1976-05-00 ·Pages 559-66

Scolnick EM, Goldberg RJ, Williams D

Abstract

The nucleic acid sequences found in DNA and RNA from rat cells which are homologous to Kirsten sarcoma virus have been characterized. The homologous sequences are present in multiple copies per diploid rat cellular genome in a variety of different rat cellular dna's. In certain cells that constitutively express only low levels of sequences homologous to Kirsten sarcoma virus, bromodeoxyuridine treatment leads to the expression of high levels of these sequences in RNA. Supernatants from cell lines producing the sequences homologous to Kirsten sarcoma virus contain high levels of these sequences which are purified to the same degree as the previously known rat type C viral nucleic acid sequences by type C particles being released from such cells. The results indicate that the sequences in rat cells homologous to Kisten sarcoma virus have three characteristics of known mammalian type C viruses, and suggest that at least part of Kirsten sarcoma virus rat-derived sequences represent a distinct class of endogenous rat type C virus that has no detectable homology to the other known class of endogenous rat type C virus.

MeSH Terms
Animals Base Sequence Bromodeoxyuridine/pharmacology Cell Line Cell Transformation, Neoplastic DNA/analysis Gammaretrovirus/analysis Nucleic Acid Conformation RNA/analysis RNA, Viral/analysis Rats Rats, Inbred Strains Retroviridae/analysis
Chemicals
RNA, Viral RNA DNA Bromodeoxyuridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scolnick E M
Goldberg R J
Williams D
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30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.18.2.559-566.1976
Published
1976-05-00
Pages
559-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515582
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.18.2.559-566.1976 PMC Full Text View journal details
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