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PMID: 6314251 Published · ppublish English Journal Article

DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites.

Nucleic acids research ·Vol. 11 ·No. 19 ·1983-10-11 ·Pages 6647-66

Everett RD

Abstract

The genes of Herpes simplex virus type 1 (HSV-1) are classified into three temporally regulated groups. The Immediate-Early (IE) genes are transcribed first by the pre-existing transcription apparatus of the cell. The Early genes are transcribed only after IE-gene expression, and finally the Late genes are activated. The control of transcription of the HSV-1 glycoprotein D (gD) gene (an Early function) was studied by quantitative S1 mapping of RNA produced in HSV-1 infected HeLa cells after short-term transfection experiments using plasmids containing the gD promoter linked to the rabbit beta-globin gene. The viral promoter in the plasmid was activated in the same way as that in the virus itself; the RNA showed a similar time-course of appearance, dependence on prior IE-gene expression and pattern of RNA cap-sites. Deletion analysis showed that the DNA sequences necessary for Early promoter activation lie within 83 bp of the RNA cap-sites in this instance. Surprisingly, a plasmid-borne beta-globin promoter was also activated by HSV-1 infection. The mechanism of this activation, and DNA sequence similarities between the promoters of HSV-1 Early and rabbit beta-globin genes are discussed.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Viral/genetics Escherichia coli/genetics Genes Genes, Viral Genetic Vectors HeLa Cells Humans Mutation Plasmids RNA, Viral/genetics Simplexvirus/genetics Transcription, Genetic Transfection Viral Envelope Proteins Viral Proteins/genetics
Chemicals
DNA, Viral RNA, Viral Viral Envelope Proteins Viral Proteins glycoprotein D, Human herpesvirus 1 DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Everett R D
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48 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.19.6647
Published
1983-10-11
Pages
6647-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326405
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.19.6647 PMC Full Text View journal details
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