Home LiteratureArticle Details
PMID: 6314261 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of the human corticotropin-beta-lipotropin precursor gene.

Nucleic acids research ·Vol. 11 ·No. 19 ·1983-10-11 ·Pages 6847-58

Takahashi H, Hakamata Y, Watanabe Y, Kikuno R, Miyata T, Numa S

Abstract

The nucleotide sequence of an 8658-base-pair human genomic DNA segment containing the entire corticotropin-beta-lipotropin precursor gene has been determined, and some sequence features of the gene and its flanking regions have been analysed. The gene is composed of 7665 base pairs including two introns of 3708 and 2886 base pairs. Comparison of the 5'-flanking sequences of the human, bovine and mouse corticotropin-beta-lipotropin precursor genes reveals the presence of a highly conserved region, which contains sequences of 14-15 base pairs homologous with sequences located upstream of the mRNA start site of other glucocorticoid-regulated genes.

MeSH Terms
Adrenocorticotropic Hormone/genetics Base Composition Base Sequence Chloramphenicol/pharmacology DNA Restriction Enzymes Dinucleoside Phosphates Genes Humans Oligonucleotides/analysis Pituitary Hormones, Anterior/genetics Plasmids/drug effects Pro-Opiomelanocortin Protein Precursors/genetics RNA, Messenger/genetics beta-Lipotropin/genetics
Chemicals
Dinucleoside Phosphates Oligonucleotides Pituitary Hormones, Anterior Protein Precursors RNA, Messenger Pro-Opiomelanocortin Chloramphenicol Adrenocorticotropic Hormone beta-Lipotropin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Takahashi H
Hakamata Y
Watanabe Y
Kikuno R
Miyata T
Numa S
References (45)
45 references, click to expand
  1. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  2. DNA sequences required for transcription in vivo of the human corticotropin-beta-lipotropin precursor gene.
    EMBO J. 1982;1(12):1533-8 PMID: 6327275
  3. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. Differential effects of hydrocortisone and tissue extracts.
    J Cell Biol. 1970 Oct;47(1):61-70 PMID: 5513559
  4. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli.
    Biochem Biophys Res Commun. 1973 Oct 15;54(4):1451-9 PMID: 4585292
  5. Mammary tumor virus induction by glucocorticoids. Characterization of specific transcriptional regulation.
    J Biol Chem. 1975 May 10;250(9):3330-6 PMID: 47326
  6. Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1816-20 PMID: 266704
  7. Pregrowth hormone messenger RNA: glucocorticoid induction and identification in rat pituitary cells.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2357-61 PMID: 267930
  8. Glucocorticoid effect on the level of corticotropin messenger RNA activity in rat pituitary.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3283-6 PMID: 198779
  9. Effects of steroid hormones on the level of corticotropin messenger RNA activity in cultured mouse-pituitary-tumor cells.
    Eur J Biochem. 1978 May;86(1):61-6 PMID: 207519
  10. Dexamethasone-mediated induction of mouse mammary tumor virus RNA: a system for studying glucocorticoid action.
    Cell. 1975 Nov;6(3):299-305 PMID: 212202
  11. Purification and characterization of the messenger RNA coding for bovine corticotropin/beta-lipotropin precursor.
    Eur J Biochem. 1979 Jan 15;93(2):213-20 PMID: 218809
  12. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.
    Nature. 1979 Mar 29;278(5703):423-7 PMID: 221818
  13. Processing of the mouse beta-globin mRNA precursor: at least two cleavage-ligation reactions are necessary to excise the larger intervening sequence.
    Cell. 1979 Aug;17(4):915-21 PMID: 487436
  14. A ubiquitous family of repeated DNA sequences in the human genome.
    J Mol Biol. 1979 Aug 15;132(3):289-306 PMID: 533893
  15. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  16. Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences.
    Nature. 1980 Mar 27;284(5754):372-4 PMID: 6244506
  17. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  18. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  19. Glucocorticoid induction of egg white mRNAs in chick oviduct.
    J Biol Chem. 1980 Aug 25;255(16):7796-800 PMID: 7400146
  20. Ovomucoid intervening sequences specify functional domains and generate protein polymorphism.
    Cell. 1980 Oct;21(3):681-7 PMID: 7438205
  21. Correlation between splicing sites within an intron and their sequence complementarity with U1 RNA.
    Cell. 1980 Oct;21(3):689-96 PMID: 6159982
  22. Sequence definition and organization of a human repeated DNA.
    J Mol Biol. 1980 Sep 25;142(3):363-86 PMID: 6257909
  23. 5'-terminal nucleotide sequence of the messenger RNA coding for bovine corticotropin/beta-lipotropin precursor.
    Eur J Biochem. 1981 Jan;113(3):531-9 PMID: 6260486
  24. Regulatory and coding potential of the mouse mammary tumor virus long terminal redundancy.
    J Virol. 1981 Jan;37(1):226-38 PMID: 6260976
  25. Identification of the mRNA coding for the ACTH-beta-lipotropin precursor in a human ectopic ACTH-producing tumor.
    Biochem Biophys Res Commun. 1981 Jan 30;98(2):535-40 PMID: 6261742
  26. The role of DNA rearrangement and alternative RNA processing in the expression of immunoglobulin delta genes.
    Cell. 1981 May;24(2):353-65 PMID: 6786756
  27. Isolation and characterization of the bovine corticotropin/beta-lipotropin precursor gene.
    Eur J Biochem. 1981 Apr;115(3):429-38 PMID: 6263630
  28. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  29. Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.
    Nucleic Acids Res. 1981 Aug 11;9(15):3719-30 PMID: 6269091
  30. Primary structure and evolution of rat growth hormone gene.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4867-71 PMID: 6946433
  31. Isolation and structural organization of the human corticotropin--beta-lipotropin precursor gene.
    FEBS Lett. 1981 Nov 30;135(1):97-102 PMID: 6274691
  32. Differential regulation by glucocorticoids of proopiomelanocortin mRNA levels in the anterior and intermediate lobes of the rat pituitary.
    Endocrinology. 1982 Apr;110(4):1442-4 PMID: 7060531
  33. Characterization of the structural gene and putative 5'-regulatory sequences for human proopiomelanocortin.
    Nature. 1982 May 27;297(5864):335-9 PMID: 6927850
  34. Structural analysis of the prolactin gene suggests a separate origin for its 5' end.
    Nature. 1982 Jun 17;297(5867):603-6 PMID: 6283362
  35. Nucleotide sequence definition of a major human repeated DNA, the Hind III 1.9 kb family.
    Nucleic Acids Res. 1982 May 25;10(10):3211-9 PMID: 6285292
  36. Asymmetrical distribution of CpG in an 'average' mammalian gene.
    Nucleic Acids Res. 1982 Dec 11;10(23):7865-77 PMID: 7155899
  37. The human pro-opiomelanocortin gene: organization, sequence, and interspersion with repetitive DNA.
    DNA. 1982;1(2):133-43 PMID: 6299668
  38. Hinf family: a novel repeated DNA family of the human genome.
    Nature. 1983 Apr 14;302(5909):587-90 PMID: 6300690
  39. Isolation and characterization of the mouse corticotropin-beta-lipotropin precursor gene and a related pseudogene.
    FEBS Lett. 1983 May 30;156(1):67-71 PMID: 6303853
  40. Subfragments of the large terminal repeat cause glucocorticoid-responsive expression of mouse mammary tumor virus and of an adjacent gene.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3637-41 PMID: 6304728
  41. DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo.
    Cell. 1983 Jun;33(2):489-99 PMID: 6190571
  42. Biological activity of cloned mammary tumor virus DNA fragments that bind purified glucocorticoid receptor protein in vitro.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:977-84 PMID: 6305596
  43. Sequence requirement for transcription in vitro of the human corticotropin/beta-lipotropin precursor gene.
    Eur J Biochem. 1983 Jul 1;133(3):599-605 PMID: 6305652
  44. The region of mouse mammary tumor virus DNA containing the long terminal repeat includes a long coding sequence and signals for hormonally regulated transcription.
    EMBO J. 1982;1(1):3-7 PMID: 6325151
  45. Repetitive DNA sequences in the human corticotropin-beta-lipotrophin precursor gene region: Alu family members.
    Nucleic Acids Res. 1982 Mar 11;10(5):1471-9 PMID: 6280146
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.19.6847
Published
1983-10-11
Pages
6847-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326418
Subset
IM
Databases
GENBANK
K02406
External Links
PubMed source DOI: 10.1093/nar/11.19.6847 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2