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PMID: 6314275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.

Nucleic acids research ·Vol. 11 ·No. 20 ·1983-10-25 ·Pages 7105-17

Elkaim R, Goding C, Kédinger C

Abstract

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection of HeLa cells, for the ability to act as a template for transcription. A region between positions -94 and -63 upstream from the major EIIa early cap site is essential both in vivo and in vitro for efficient promoter function. By cotransfection of the EIIa deletion mutants with the EIa transcription unit it has been possible to demonstrate that deletion to position -94 does not affect induction of transcription of the EIIa early gene by the EIa transcription unit, but deletion to position -63 results in loss of detectable levels of EIIa early specific RNA. Thus, sequences upstream from position -94 of the EIIa early gene are not involved in the induction of the EIIa early gene by the EIa transcription unit.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence DNA Restriction Enzymes Genes, Viral HeLa Cells/metabolism Humans Mutation Operon Templates, Genetic Transcription, Genetic Transfection
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elkaim R
Goding C
Kédinger C
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.20.7105
Published
1983-10-25
Pages
7105-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326441
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.20.7105 PMC Full Text View journal details
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