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PMID: 6315589 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Surface localization of Pseudomonas aeruginosa outer membrane porin protein F by using monoclonal antibodies.

Infection and immunity ·Vol. 42 ·No. 3 ·1983-12-00 ·Pages 1027-33

Mutharia LM, Hancock RE

Abstract

Hybridomas secreting highly specific monoclonal antibodies against porin protein F of Pseudomonas aeruginosa were isolated. These antibodies interacted with protein F in outer membranes isolated from strains representing the 17 serotypes of P. aeruginosa and from another 15 clinical isolates from patients with cystic fibrosis. The cell surface localization of antigenic sites on protein F was shown by indirect immunofluorescent techniques with these monoclonal antibodies. No fluorescence was observed on a protein F-deficient strain H283 of P. aeruginosa. Another monoclonal antibody specific for outer membrane lipoprotein H2 of P. aeruginosa showed no fluorescence on intact, wild-type bacterial cells, but was able to interact with a rough, LPS-deficient mutant.

MeSH Terms
Antibodies, Monoclonal/immunology,isolation & purification Antibody Specificity Antigens, Bacterial/analysis Antigens, Surface/analysis Bacterial Outer Membrane Proteins Bacterial Proteins/analysis Cystic Fibrosis/microbiology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Humans Hybridomas/immunology Membrane Proteins/analysis Porins Pseudomonas aeruginosa/immunology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Proteins Membrane Proteins Porins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mutharia L M
Hancock R E
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.42.3.1027-1033.1983
Published
1983-12-00
Pages
1027-33
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264403
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.42.3.1027-1033.1983 PMC Full Text View journal details
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