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PMID: 387596 Published · ppublish English Journal Article

Immunization with major outer membrane proteins in experimental salmonellosis of mice.

Infection and immunity ·Vol. 25 ·No. 3 ·1979-09-00 ·Pages 857-62

Kuusi N, Nurminen M, Saxen H, Valtonen M, Mäkelä PH

Abstract

Porin (outer membrane protein) preparations extracted from a rough (Rb2) mutant of Salmonella typhimurium proved to be good immunogens in mice and rabbits. The antibody response achieved was measured by using enzyme-linked immunosorbent assay techniques. High titers of both antiporin and antilipopolysaccharide were detected in both species. The rabbit antiserum raised against the porins and the porin preparations themselves had a highly significant protective capacity against intraperitoneal Salmonella infection of mice. Absorption of the rabbit antiporin serum with lipopolysaccharide immunosorbent did not change its protective capacity in a passive immunization experiment, suggesting that the antiporin antibody preparations were the active components.

MeSH Terms
Animals Antibodies, Bacterial/biosynthesis Antigens, Bacterial/immunology Bacterial Proteins/immunology Immunization, Passive Lipopolysaccharides/immunology Membrane Proteins/immunology Mice Salmonella Infections, Animal/immunology Salmonella typhimurium/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins Lipopolysaccharides Membrane Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kuusi N
Nurminen M
Saxen H
Valtonen M
Mäkelä P H
References (22)
22 references, click to expand
  1. THE PRESENT STATUS OF FIELD AND LABORATORY STUDIES OF TYPHOID AND PARATYPHOID VACCINES WITH SPECIAL REFERENCE TO STUDIES SPONSORED BY WORLD HEALTH ORGANIZATION.
    Bull World Health Organ. 1965;32:29-36 PMID: 14290077
  2. Statistical aspects of the analysis of data from retrospective studies of disease.
    J Natl Cancer Inst. 1959 Apr;22(4):719-48 PMID: 13655060
  3. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  4. Pneumococcal infection and pneumococcal vaccine.
    N Engl J Med. 1977 Oct 27;297(17):938-9 PMID: 20576
  5. Artificial Salmonella vaccines: O-antigenic oligosaccharide-protein conjugates induce protection against infection with Salmonella typhimurium.
    Infect Immun. 1979 Sep;25(3):863-72 PMID: 387597
  6. Protective effects of a supernatant factor from Salmonella typhimurium on Salmonella typhimurium infection of inbred mice.
    Infect Immun. 1978 Oct;22(1):125-31 PMID: 365739
  7. Experimental gram-negative bacterial sepsis: reevaluation of the ability of rough mutant antisera to protect mice.
    Proc Soc Exp Biol Med. 1978 Jul;158(3):482-90 PMID: 356058
  8. The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions.
    Annu Rev Biochem. 1978;47:481-532 PMID: 354502
  9. Mouse virulence of Salmonella typhimurium mutants deficient in two major outer membrane proteins.
    Infect Immun. 1977 Nov;18(2):454-8 PMID: 336546
  10. Immunity to experimental Salmonella infection: studies on the protective capacity and immunogenicity of lipopolysaccharide, acetone-killed cells, and ribosome-rich extracts of Salmonella typhimurium in C3H/HeJ and CD-1 mice.
    J Immunol. 1978 Sep;121(3):1010-4 PMID: 690428
  11. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
    J Biol Chem. 1974 Jan 25;249(2):634-44 PMID: 4129205
  12. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
    J Immunol. 1972 Jul;109(1):129-35 PMID: 4113792
  13. Immunization with R mutants of S. Minnesota. I. Protection against challenge with heterologous gram-negative bacilli.
    J Immunol. 1972 Mar;108(3):601-10 PMID: 4622185
  14. The protective role of proteins from Salmonella typhimurium in infection of mice with their natural pathogen.
    Rev Immunol (Paris). 1972 Apr-Jun;36(3):77-84 PMID: 4633830
  15. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.
    Immunochemistry. 1969 Jan;6(1):43-52 PMID: 4975324
  16. Mouse virulence of Salmonella strains: the effect of different smooth-type O side-chains.
    J Gen Microbiol. 1970 Dec;64(3):255-68 PMID: 4930444
  17. Protection against group B meningococcal disease. I. Comparison of group-specific and type-specific protection in the chick embryo model.
    J Exp Med. 1976 Aug 1;144(2):319-29 PMID: 822114
  18. Bacteriophage-resistant mutants of Salmonella typhimurium deficient in two major outer membrane proteins.
    J Bacteriol. 1976 Aug;127(2):941-55 PMID: 783123
  19. Functional role of antibody against "core" glycolipid of Enterobacteriaceae.
    J Clin Invest. 1975 Oct;56(4):850-61 PMID: 1099119
  20. Outer membrane of Salmonella. Isolation of protein complex that produces transmembrane channels.
    J Biol Chem. 1976 Apr 10;251(7):2176-8 PMID: 773934
  21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  22. A new method for the extraction of R lipopolysaccharides.
    Eur J Biochem. 1969 Jun;9(2):245-9 PMID: 5804498
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.25.3.857-862.1979
Published
1979-09-00
Pages
857-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414527
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.25.3.857-862.1979 PMC Full Text View journal details
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