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PMID: 6323757 Published · ppublish English Journal Article

Biochemical map of polypeptides specified by foot-and-mouth disease virus.

Journal of virology ·Vol. 50 ·No. 2 ·1984-05-00 ·Pages 579-86

Grubman MJ, Robertson BH, Morgan DO, Moore DM, Dowbenko D

Abstract

Pulse-chase labeling of foot-and-mouth disease virus-infected bovine kidney cells revealed stable and unstable viral-specific polypeptides. To identify precursor-product relationships among these polypeptides, antisera against a number of structural and nonstructural viral-specific polypeptides were used. Cell-free translations programmed with foot-and-mouth disease virion RNA or foot-and-mouth disease virus-infected bovine kidney cell lysates, which were shown to contain almost identical polypeptides, were immunoprecipitated with the various antisera. To further establish identity, some proteins were compared by partial protease digestion. Evidence for a membrane association of the polypeptides coded for by the middle genome region is also presented. A biochemical map of the foot-and-mouth disease virus genome was established from the above information.

MeSH Terms
Animals Aphthovirus/genetics Cattle Cell Line DNA-Directed RNA Polymerases/genetics Genes Genes, Viral Kidney Kinetics Peptides/genetics,isolation & purification Protein Biosynthesis RNA, Viral/genetics Rabbits Reticulocytes/metabolism Viral Proteins/genetics,isolation & purification Virion/genetics
Chemicals
Peptides RNA, Viral Viral Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Grubman M J
Robertson B H
Morgan D O
Moore D M
Dowbenko D
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48 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.2.579-586.1984
Published
1984-05-00
Pages
579-86
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255676
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.50.2.579-586.1984 PMC Full Text View journal details
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