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PMID: 6324096 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterisation of the rRNA genes from the human malaria parasite Plasmodium falciparum.

Nucleic acids research ·Vol. 11 ·No. 24 ·1983-12-20 ·Pages 8703-17

Langsley G, Hyde JE, Goman M, Scaife JG

Abstract

Ribosomal DNA fragments from the human malaria parasite Plasmodium falciparum have been cloned and analysed in detail. Restriction mapping shows that the cloned fragments are different. However, they do have some similarities, in particular a small stretch of A+T-rich DNA located between the small and large subunit rRNA genes. A small rRNA gene has been mapped to this A+T-rich region. Copy number analysis reveals that each fragment is represented approximately 4 times in the genome, and implies that there are a total of 8 rRNA genes organised into at least two classes of transcription unit. Analysis of a third overlapping rDNA fragment indicates that the large subunit rRNA gene of at least one transcription unit contains an intervening sequence.

MeSH Terms
Animals Base Composition Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Genes Nucleic Acid Hybridization Plasmodium falciparum/genetics RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Langsley G
Hyde J E
Goman M
Scaife J G
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.24.8703
Published
1983-12-20
Pages
8703-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326618
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.24.8703 PMC Full Text View journal details
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