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PMID: 6324128 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the gene for the P680 chlorophyll alpha apoprotein of the photosystem II reaction center from spinach.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2837-50

Morris J, Herrmann RG

Abstract

The DNA sequence of 2210 nucleotides including the gene for the "51 kd" chlorophyll alpha-conjugated thylakoid membrane protein associated with the photosystem II reaction center of spinach has been determined. This protein is functionally identical with the P680 chlorophyll alpha apoprotein that catalyses the primary light-induced photochemical processes of photosystem II (Camm, E.L. and Green, B.R. (1983) Biochim. Biophys. Acta 724 291-293). The only large open reading frame in the sequence consists of 508 triplets encoding a protein of molecular mass of 56,246 kd. The deduced amino acid sequence shows clustering of hydrophobic residues into seven core regions which probably traverse the membrane, and a large hydrophilic domain of about 200 amino acids interspersed between span VI and VII. Potential transcription promotor and terminator signals flanking the structural gene show prokaryotic-like features. Seven discrete RNA species ranging in size from 2.0 to over 5.0 kilobases display complementarity to apoprotein coding sequences implying that the region can be polycistronically transcribed. The primary transcript includes information for at least two further genes coding for subunits of the cytochrome b/f complex.

MeSH Terms
Amino Acid Sequence Apoproteins/genetics Base Sequence DNA Restriction Enzymes Genes Light-Harvesting Protein Complexes Membrane Proteins/genetics Nucleic Acid Conformation Nucleic Acid Hybridization Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics Plants/metabolism Plasmids
Chemicals
Apoproteins Light-Harvesting Protein Complexes Membrane Proteins Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morris J
Herrmann R G
References (29)
29 references, click to expand
  1. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  2. Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6903-7 PMID: 16593250
  3. Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6260-4 PMID: 16593238
  4. The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA.
    Nucleic Acids Res. 1981 Jul 24;9(14):3251-70 PMID: 6269077
  5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  6. Spinach chloroplast mRNA for a 32 000 dalton polypeptide: size and localization on the physical map of the chloroplast DNA.
    Biochim Biophys Acta. 1980 Dec 11;610(2):297-310 PMID: 7213628
  7. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  8. Molecular Basis of Herbicide Resistance in Amaranthus hybridus.
    Science. 1983 Dec 23;222(4630):1346-9 PMID: 17773338
  9. Nucleotide sequence surrounding a ribonuclease III processing site in bacteriophage T7 RNA.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):984-8 PMID: 265592
  10. The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.
    Gene. 1982 Nov;20(1):91-102 PMID: 7160620
  11. Genes and transcripts for the polypeptides of the cytochrome b6/f complex from spinach thylakoid membranes.
    EMBO J. 1983;2(6):979-86 PMID: 16453463
  12. Amplification and characterization of a beta-globin gene synthesized in vitro.
    Cell. 1976 Jun;8(2):163-82 PMID: 61066
  13. Nucleotide sequence of soybean chloroplast DNA regions which contain the psb A and trn H genes and cover the ends of the large single copy region and one end of the inverted repeats.
    Nucleic Acids Res. 1983 Oct 25;11(20):7157-67 PMID: 6314279
  14. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  15. Nucleotide sequence of the gene for the M(r) 32,000 thylakoid membrane protein from Spinacia oleracea and Nicotiana debneyi predicts a totally conserved primary translation product of M(r) 38,950.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7699-703 PMID: 16593262
  16. Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii.
    Cell. 1978 Oct;15(2):661-70 PMID: 719757
  17. Mr-values of mature subunits I and III of beef heart cytochrome c oxidase in relationship to nucleotide sequences of their genes.
    FEBS Lett. 1981 Nov 30;135(1):164-6 PMID: 6274685
  18. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  19. The complete nucleotide sequence of 16S ribosomal RNA gene from tobacco chloroplasts.
    Gene. 1982 Feb;17(2):213-8 PMID: 7044901
  20. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  21. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  22. Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.
    J Mol Biol. 1975 Dec 15;99(3):419-43 PMID: 765476
  23. Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):111-5 PMID: 16592604
  24. Sequence of the intercistronic region between the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit and coupling factor beta subunit gene.
    Nucleic Acids Res. 1982 Aug 25;10(16):4923-34 PMID: 6290996
  25. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  26. Maize plastid gene expressed during photoregulated development.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3060-4 PMID: 16592541
  27. Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA.
    Nucleic Acids Res. 1982 Apr 24;10(8):2665-76 PMID: 6281739
  28. Localization of the genes for the two chlorophyll a-conjugated polypeptides (mol. wt. 51 and 44 kd) of the photosystem II reaction center on the spinach plastid chromosome.
    EMBO J. 1983;2(12):2229-37 PMID: 16453486
  29. The maize chloroplast genes for the beta and epsilon subunits of the photosynthetic coupling factor CF1 are fused.
    Nucleic Acids Res. 1982 Aug 25;10(16):4985-5002 PMID: 6290998
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.6.2837
Published
1984-03-26
Pages
2837-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318709
Subset
IM
Databases
GENBANK
X00471
External Links
PubMed source DOI: 10.1093/nar/12.6.2837 PMC Full Text View journal details
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